Nowadays about 100 million of married couples all over the world, are infertile. The problem affects the demographic situation in each country. Ukraine is not an exception. According to the State Statistics Committee the birth rate in the country dropped down from 13.3 to 8.1, which contributed to the aging of the nation.

When speaking about the structure of infertile marriages, it should be noted that a weighty contribution falls on male infertility. In turn, among the leading causes of male infertility there is a large portion of secondary androgen deficiency (SAD). First of all, the deficiency is associated with decrease of testosterone level, which is the key element to ensure normal androgenic status of a man. Therefore, the treatment of SAD is primarily based on the application of testosterone replacement therapy. This therapy is one of the most common and effective treatments. Nevertheless, testosterone replacement therapy is known to cause a lot of side effects. Firstly, this therapy requires constant use of medication. Secondly, there is a greater increase of cancer risk, particularly, prostate cancer. Another drawback of androgen replacement therapy is high treatment costs.

Thus, the development of effective treatments is apparently needed, that would not have the side effects and their cost estimate would be much more beneficial compared with pharmacological methods.

During our studies we proved possible stem cells usage for treatment of SAD. The first step of the studies was modeling relevant pathology in rats, which was reproduced with CdCl2. It was experimentally proved that the use of xenobiotics leads to loss of relevant functions in testicular endothelial cells, specifically, the ability to develop into mature sperm. Our goal was to choose the optimal dose of xenobiotics for creating irreversible SAD and to investigate its impact on the mass of target organs. The results of the experiment on the selection of an effective dose of CdCl2 to the modeling of secondary androgen deficiency (SAD) in rats to further explore the possibility of stem cells culture for treatment by SAD. In the experiment animals were injected doses of xenobiotic, 0,55; 1,0; 1,5 and 2,0 mg/kg of animals body weight. On the background of the affected animal’s xenobiotic measured the mass of the target organs and masses of animals on the 7th, 14th and 28th days of the experiment. The introduction of animal xenobiotic dose 0,55 mg/kg body weight of animals on the 28th day of the experiment differed from the intact groups (IG), only 2,9 g, the weight of the testes - 20,2 mg, the mass of the ventral part of the prostate (VPP) – at 32,8 mg, weight of seminal vesicles – at 11,4 mg, the mass of the epididymis – at 40,5 mg. In the application of the xenobiotic dose of 1,0 mg/kg weighing on the 28th day gave the following result: mass of animals was lower than in the IG on 27,8 g, the mass of the testes – at 235,2 mg weight VPP – at 56,1 mg seed weight bubbles – at 32,6 mg, the mass of the epididymis – at 82,9 mg. In the application of the xenobiotic dose of 1,5 mg/kg of weighing and animals gave the following result: mass of animals was lower than in the IG at 46,0 g, the weight of the testes - at 388,4 mg, weight VPP – to 358,5 mg, the weight of seminal vesicles – at 77,1 mg, weight of the epididymis – at 169,6 mg. When using doses of 2,0 mg/kg CdCl2 up to 28th days of the experiment none animal survived. Thus, on the basis of the experiments it was concluded upon worthiness and feasibility of using in subsequent experiments, the xenobiotic in the dose 1,5 mg/kg body weight of animals in order to play the model SAD.

Keywords: target organs, secondary androgen deficiency, the culture of stem cells

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