Activity of free radical oxidation plays an important role in the genesis and course of many diseases. Increase of reactive oxygen species leads to destruction of cell membranes and its death in different pathology. Accumulation of toxic products of lipid peroxidation (LP) occur due to oxidative stress. As a result disturbance of cellular homeostasis, metabolic disorders, changes of immune status occur.

During recent years attention of researchers is centered around the role of lipid peroxidation in the development of various pathologies: cardiovascular, lung diseases, allergies and other. Exogenous allergic alveolitis has a special place among allergic diseases.

Etiological factors of the disease are already known today, but its pathogenesis has not been completely clarified. So, the aim of our work was to investigate the functional state of some indicators of prooxidant and amtioxidant systems in condition of experimental allergic alveolitis development and define the corrective effect of thiotriazolin on these indicators. The results of biochemical investigations have shown that in animals in condition of experimental allergic alveolitis there are typical signs of oxidative stress development.

This is reflected in the considerable increasing of activity of oxidative reactions and as a result excessive accumulation of free radicals in trachea takes place. Thus, level of dienic conjugatis in trachea of guinea pigs increased on the 34th day, next, on the 44th day there have not been any significant changes but in late period of the experiment (54 day) it increased more in comparison with the first group of animals. Learning of another indicator content that characterizes prooxidant system - malonic dialdegide revealed that during early periods of the experiment development (34th, 44th days) its content does not have any significant changes and remains at the values of the first group, only later, on the 54th day of the disease, the level of malonic dialdegide increased comparing with the group of animals with allergic alveolitis on the 24th day. Increasing of primary product of lipid peroxidation- dienic conjugatis in the early periods but secondary- malonic dialdegide only in the late period of experimental allergic alveolitis development is a sign of important role of compensatory mechanisms which are directed on neutralization of excessive amounts of lipid peroxidation products and regulation of cellular homeostasis.

Functional state of antioxidant system was learned by means of superoxyddismutase and catalase. Activity of superoxyddismutase in biomaterial was on the level of the first group of animals in different periods of allergic alveolitis (34, 44 days). This can be regarded as sufficient level of activity for prevention of excessive activation of LP. Decreasing of this indicator activity occurs only on the 54th day in trachea of animals as manifestation of compensatory mechanisms exhausition. Investigation of catalase enzyme established that changes in its dynamics were intensive with tendency to increasing. Its reduction has been steady during all days (24, 34, 44, 54) of the experimental model of disease formation.

Usage of thiotriazolin with its antioxidant action has led to reduction of dienic conjugatis and malonic dialdegide content in trachea of guinea pigs and increase of superoxyddismutase and catalase in comparison with guinea pigs without received treatment on the 54th day.

Thus, in pathogenesis of experimental allergic alveolitis disturbance of balance between prooxidant and antioxidant systems occurs. Increase of free radicals synthesis on base of exhaustion of compensatory reactions leads to oxidative stress development. Usage of thiotriazolin has a corrective effect on the disturbed indicators.

Keywords: experimental allergic alveolitis, lipid peroxidation, antioxidant system, thiothriasolin

Full text: PDF (Ukr) 188K