A method of chelated zinc revealing in the cells was elaborated by using highly selective luminescent reaction of 8-(p-toluenesulfonylamino)-quinoline (8-TSQ). Quantitative value of this reaction was evidenced by absence of metachromazy and fading of agents under the influence of ultraviolet rays, submitting to the laws of Buger, Lambert and of Beer. Positive cytochemical 8-TCQ reaction with zinc was revealed as yellow-green luminescence in the cells of hippocampus, pancreatic islets, epiphysis, thymus. Light filter V-1 was used for luminescence exiting and the Y-18 as a protective one.

Pieces of hippocampus, epiphysis, thymus and pancreas were taken from animals and fixed in cooled 70° alcohol, saturated with H2S. Then the pieces were soaked in alcohol of increasing concentration, transferred in two xilols, mixture of xilol and paraffin, two liquid kinds of paraffin and flooded in paraffin.

The sections were deparaffinized by tilling in xilol and alcohol, fluorochromized by alcohol solution of 8-TCQ, washed with hot solution of Natrium hydroxide, dried in air, placed in to glycerine and examined under luminescent microscope (light filters V-1 and Y-18). On the agents yellow-green luminescence was revealed in the cells of Fascia dentata, CA2-CA4 of hippocampus, the cells of epiphysis, thymus and B-insulocytes.

On the example of pancreatic B cells it was shown that fasting of the animals induced lowering of glycemia, increasing of zinc and insulin content in these cells. On the contrary glucose load led to glycemia rise, fall of zinc and insulin content in islet B-cells. Diabetogenic agent injection induced the glycemia rise and zinc deficit of islet B-cells. Positive correlation of zinc and insulin level changes in B-insulocytes indicates functional connection of both components in them.

The data obtained permit to infer that zinc content in the cells may be an indicator of their functional state. Such indicator was used for the evidence of functional connections between hippocampus, pancreatic islets, epiphysis and thymus.

It was shown, that fasting of animals induced the increase of zinc content in the cells of hippocampus epiphysis, thymus and B-insulocytes. Positive correlation of zinc content changes was noticed in all cell types. Glucose load led to the contrary results: zinc content was decreased in all cell types indicated. Alloxan injections induced significant decrease of this metal concentration in the cells.

Thus, zinc content alterations in hippocampus, pinealocytes and thymocytes are similar and dependent on insular apparatus functional state.

The results of investigations permit to formulate a conception according to which hippocampus regulates insulin content in pancreatic islets through the change of epiphysis and thymus function.

Keywords: epiphysis, hippocampus, pancreatic islets, thymus, zinc

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