Long-term suppression of gastric acid secretion is associated with a range of negative consequences for the gastrointestinal tract (GIT). One of them is dysbiosis development that leads to colonization of GIT by opportunistic microbiota, thus favoring inflammatory processes. Oxidative stress is a terminal non-specific stage of duodenal cells damage in these conditions. Cure of dysbiosis with probiotics can potentially restore the pro-/antioxidative balance of duodenal cells upon long-term inhibition of gastric acid secretion.

The goal of investigation was to determine the effect of multiprobiotic Symbiter on the intensity of free-radical processes in rat duodenal epitheliocytes upon long-term gastric hypochlorhydria.

Experiments were carried out on white non-strain male rats. Animals were divided into 4 groups. First group (control) obtained water (0,2 ml abdominally and 0,5 ml per os), second – multiprobiotic “Symbiter” per os (0,14 ml/kg) during 28 days, third – omeprazole (abdominally 14 mg/kg), and fourth group along with omeprazole obtained “Symbiter” at the same dose.

Duodenal cells were isolated by low-temperature method with following separation of crypt and villus cells. The level of diene conjugates, TBA-reactive substances, superoxide anion as well as superoxide dismutase, catalase and xanthine oxidase activity were determined by spectrophotometric assays, while Shiff bases amount – by fluorimetry. The significant violation of pro-/antioxidative balance in crypts and villus cells upon hypochlorhydria (third group) was established. It was associated with increased levels of superoxide anion and lipid peroxidation products, as well as with elevated xanthine oxidase and superoxide dismutase activity on the back of decreased catalase activity. It should be noted, that level of oxidative damages in crypt cells was higher than in villus epitheliocytes upon these conditions. All above mentioned indices were closer to control values in animals treated simultaneously with omeprazole and Symbiter (fourth group) in comparison with animals of the second group. There were no statistically significant differences between parameters of villus cells of the animals from second group and control ones, while most of determined indices of crypt cells were lower than in animals from the first group.

It was established that parameters of pro-/antioxidative balance of duodenal cells (both villus and crypt) were similar to the control values in the case of simultaneous administration of multiprobiotic Symbiter and omeprazole. So, it can be suggested that this preparation is able to counteract the development of oxidative damages of duodenal epitheliocytes upon long-term hypoacidic states. Dysbiotic pathway plays a significant role in the development of oxidative stress in duodenal epithelial cells, since its treatment with multiprobiotic is associated with amelioration of above mentioned parameters. This evidence serves as a basis to recommend multiprobiotic preparations as a support factor upon prevention of oxidative damage of duodenal cells upon long-term hypochlorhydria.

Keywords: hypochlorhydria, duodenum, oxidative stress, probiotics

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