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ECPB 2013, 62(2): 107–111
Assisting a doctor

Laboratory control of vectors – key link of natural foci monitoring of the tick-borne transmission zooanthroponozes

VYNOGRAD N., KOMARENKO N.
Abstract

The article is dedicated to the issue of the circulation of causative agents of tick-borne transmissible extremely dangerous pathogens in different landscape-climatic areas of Kyiv Region. The floro-faunistical and climatic-geographical conditions of Kyiv Region are favorable for occurring of natural foci of tick-borne transmission diseases. Kyiv Region is located on two different landscape zones – Polissya in northern part and Forest-Steppe in southern part of the administrative territory.

By using complex of modern bacteriological, serological, molecular-genetic methods ticks from biotic and abiotic objects on different landscape zones were collected and investigated. The aim of the laboratory monitoring was to detect antigens or/and genes of rabbit-fever, Q-fever, ixodes tick-borne borreliosis, and tick-borne encephalitis.

It was set that the most widespread natural foci disease from the marked above was an ixodes tick-borne borreliosis, according to the rate of infected vectors as Ixodes ricinus, Dermacentor reticulatus, and Dermacentor marginatus. All administrative territories of the area have been found endemic at ixodes tick-borne borreliosis. For the first time it was shown by PCR method of circulation in this area of Borrelia afzelii and Borrelia garinii, here in 11,1% of tests of Ixodes ricinus both species were detected.

Actual in the area is tick-borne encephalitis, that circulates among Ixodes ricinus, Dermacentor reticulatus, and Dermacentor marginatus. Special disturbance is caused by circumstances where the greatest part of positive, in relation to tick-borne encephalitis of ticks, was revealed in collections from cattle – 29,87±1,54%, from domestic dogs – 26,67±2,64%.

For the first time circulation of Ñoxiella burnetii in this area was detected. Specific species of tick which provide transmission of causative agent were certain as main vector Dermacentor reticulatus, and additionally Ixodes ricinus was detected.

The rabbit-fever as endemic for Kyiv Region remained. Antigens and genomes of causative agent were detected in the ticks of Dermacentor reticulatus (main vector) and Dermacentor marginatus (additional vector) but not Ixodes ricinus on Polissya and Forest-Steppe landscape zones.

At parallel investigations for the presence of the ticks in the tramsmission agents simultaneous presence of antigens of two causative agents was educed: Francisella tularensis – Tick-borne encephalitis, Francisella tularensis – Ñoxiella burnetii; and three causative agents: Francisella tularensis – Tick-borne encephalitis – Borrelia burgdorferi s.l, Francisella tularensis – Tick-borne encephalitis – Ñoxiella burnetii.

Keywords: zooanthroponozes, natural foci, Ixodes-fauna

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