Biochemical researches results revealed disruption of lipid peroxidation and antioxidant status in bronchoalveolar washings (BAW) in different periods of pneumonia forming, both before and after the application of antioxidant corvitin.
Among lipid peroxidation indicators malonic dialdehyde is one of the most important – it is the end product of lipid peroxidation and its share is 40% of all lipid products and dien conjugates – are the primary products of BPO.v The results of the research conducted have demonstrated that on the first day the DC and MDA content in broncho-alveolar washings of staphylococcus infected animals did not change significantly in comparison with indicators of healthy guinea pigs group. Later, on the third day of experimental pneumonia, one could experience a slight increase in DC content of 23,8% (p<0,05) and MDA of 17,6% (p<0,05) in BAW compared with the controls. Then, on the fifth day of the experiment, gradual increase in control of 28,5% (p<0,05) took place and MDA of 17,7% (p<0,05) and in the latest period (on the seventh day) of EP these indicators gained the biggest changes: DK increased of 33,8% (p<0,05), and MDA of 23,5% (p<0,05) in bronchoalveolar washings in comparison with the first group of animals.
Thus, the increase in DC and MDA in broncho-alveolar washings in experimental pneumonia in guinea pigs indicates a gradual excessive formation of lipid peroxidation products, depending on the duration of the inflammatory process in the lungs. According to the results of the research conducted, an excessive radical formation caused violation of antioxidant protection. However, in the early period of VC formation (the first day) it was found that the activity of SOD and CT in bronho-alveolar washings did not change significantly. These indicators were at the control level.
On the third day of EP an activity of superoxide dismutase (SOD) inhibited by 12,6% (ð<0,05) and catalase by 15,3% (ð<0,05) in the first group against BAW animals. Later, on the fifth day of EP, an activity of SOD further reduced to 19,6% (ð<0,05) and CT to 25,1% (ð<0,05) in BAW compared with the intact group of animals, indicating AOC depression.
Late period, which included the seventh day of the experiment in broncho-alveolar washings, was characterized by significant changes in antioxidant defense system, including SOD activity is inhibited by 22,6% (ð<0,05), and the activity of CT by 27,2% (ð<0,05) compared with the I-st group of animals.
Thus, the research of indicators of lipid peroxidation and antioxidant system in BAW males with EP indicates an excessive accumulation of lipid peroxidation products (on the 3rd, 5th and 7th day), which causes AOS inhibition, that is unable to neutralize.
The use of corvitini during 7-day period in 40 mg/kg intraperitoneum dose led to reduced control to 14,5% (ð<0,05) and MDA to 19,0% (ð<0,05) and increase in SOD in 19,6% (ð<0,05) and CT in 16,8% (ð<0,05) in BAW compared with the group of guinea pigs with EP that were not treated with this drug – the fact that gives reasons to state the corrective effect of the parameters studied.
Conclusion. EP is accompanied by an excessive accumulation of lipid peroxidation products against enzymatic deficiency link to AOS before treatment with corvitini medication led to corrective effect on affected metabolic processes in the conditions of inflammation in the lungs.
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