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ECPB 2015, 71(3): 47–55
Clinical physiology and biochemistry

Biometric analysis of prooxidant-antioxidant balance for the actions of helium-neon laser radiation and ozone physiological fluid in the blood of patients with pulmonary tuberculosis against the background of chemoth


Development of pulmonary tuberculosis is accompanied by oxidative stress which is typical for the intensity of free radical processes and decrease of natural antioxidant enzymes.

Progression of pulmonary tuberculosis accompanied by significant activation of free radical processes and lipid peroxidation products release into the bloodstream that promote blood clotting, increase adhesion and aggregation of blood cells, increasing its viscosity, leading to complications and increase the severity of the disease [11]. This makes the search for new treatments for patients with pulmonary tuberculosis using physical and chemical means countervailing action.

Today we know that helium-neon laser radiation and ozone physiological solution is an effective non-medicated therapeutic agent in the treatment of many diseases. Helium-neon laser radiation has a stimulating effect on the structure of cell membranes, modulates antioxidant factors in cells that ultimately improves the reparative processes [3, 12].

One of the leading biological effects of ozone (О3) is to optimize the metabolic processes in the body due to the normalization of the balance of pro- and antioxidant systems, facilitating the synthesis of arachidonic acid and prostoglandin that reduce tissue hypoxia and adjust electrolytic balance that is essential for pulmonary tuberculosis [1, 19–22]. The starting material studies were experimental data [10, 15, 16], namely the average values of diene conjugates (DC) and TBA-positive products (TBA-PP), peroxide hemolysis of red blood cells (PHE), total oxidant activity of blood plasma (ZOA) molecules of middle mass (MMM) and indicators of activity of antioxidant enzymes superoxide dismutase (SOD) and catalase (K), protein ceruloplasmin (CP) for the actions of He-Ne laser radiation, ozone physiological solution and their combined effects.

The tested material worked on by cluster and univariate analysis of variance. Using cluster analysis incrementally analyzed the distance between the observations and clusters are created for the studied parameters and create a new cluster and steam, which was the smallest distance.

Evaluation of the combined effect of helium-neon laser (with frequency 1,0–3,0 kHz, 5,0 kHz, 100 kHz and without them), ozonized saline with ozone concentration of 8 mg/L ↑↓ and 12 mg/L ↑↓ (in increments of 2 mg/L), and combinations there of processes lipid peroxidation, antioxidant enzyme activity and the level of endogenous intoxication in patients with destructive pulmonary tuberculosis in the intensive phase of standardized chemotherapy using cluster and univariate analysis of variance.

The results suggest a positive effect of He-Ne laser (with frequency 1,0–3,0 kHz, 5,0 kHz, 100 kHz and without them), ozonized saline (with varying concentrations of О3) and their combined impact on the prooxidant-antioxidant balance in the blood of patients with pulmonary tuberculosis compared to one HT leads to reduce the intensity of lipid peroxidation and antioxidant protection enzymes increased activity in the blood of patients with pulmonary tuberculosis.

The optimum effect of ozonized saline (О3 concentration of 12 mg/L ↑, 12 mg/L ↑↓, the number of procedures 14, 18) and the combined influence of О3 (12 mg/L ↑ and 12 mg/L ↑↓, the number of procedures 14, 18) intravenous blood irradiation with frequency 100,0 kHz and 5,0 kHz on the background of chemotherapy according to the prooxidant-antioxidant balance.

Keywords: lipid peroxidation, antioxidant protection, helium-neon laser radiation, ozone physiological fluid, tuberculosis

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