The effect of different doses of Germanium citrates obtained by nanotechnology method and chemical synthesis on growth and development of F1 female rats organism`s before fertilization and during pregnancy also their reproductive ability was investigated.

It is established that the adding nanogermanium citrate to drinking water for female rats during the milk period, physiological and sexual maturation and pregnancy in doses of 10 (II), 20 (III), 200 (IV group) mcg Ge/kg body weight and 2 mg Ge chemically synthesized Germanium citrate (V group), stimulated growth and development of their organism in all experimental groups. The highest rates of body weight of females at the age of 70–110 days were in the third group (р < 0,001), compared with the control (I) group. It was noted the stimulating effect of applied doses Germanium on the development of the respiratory organs, as evidenced by the significantly higher levels of lung weight coefficients in females of all experimental groups. More expressed this effect was observed in animals the second and third groups for which also was noted a significantly higher kidneys weight coefficient. In the blood of pregnant females of the second and third groups was observed the increasing number of red blood cells and hematocrit index (р < 0,001; р < 0,005). The hemoglobin content and the number of white blood cells, including lymphocytes and monocytes, were increased in the blood of females of the second, third and fifth groups, when granulocyte – only for rats of the second group. The obtained results point to a pronounced effect of Germanium citrate on the reproductive capacity of female of the fourth and fifth research groups, which is characterized by intergroup differences of number of corpus luteum, implantation sites, live fetuses and embryo mortality. In the female of fifth group established a higher level of overall embryo mortality (29.2 %) compared to the embryos of control (14 %) also third – 11.1 % and fourth – 9.1 % groups. The pre-implantation embryo mortality was 2 (4.2 %) in fifth, 3 (6.8 %) – fourth, 5 (11.1 %) – third and 8 (14 %) – control (I) groups. The postimplantation embryo mortality were noted only for the fourth (1 or 2.4 %) and fifth (12 or 26 %) groups, which significantly affected on the number of received fetuses. The lowest this rate were established in fifth group (34 or 6.8/female) compared with third and fourth (40 or 10 fetuses/female) and control (49 or 9.8/ female) groups. Significant difference between the number of live fetuses in females of other research groups or implantation sites and corpus luteum of all experimental groups compared to intact (I) group were not set. Thus, prolonged watering F1 female rats with different doses of Germanium citrate obtained with using nanotechnology or by chemical methods, leads to a pronounced biological effects, which characterized by significant changes in parameters hematopoietic function and reproductive ability of the organism during pregnancy, lower embryonic mortality by influence 20 and 200 mcg nanogermanium but higher – at a dose of 2 mg Ge, obtained by chemical synthesis.

Received 24.11.2016

Keywords: rats, Germanium citrate, blood, embryos

Full text: PDF (Ukr) 1.00M

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