The problem of treatment of patients with chronic periodontitis forms of permanent teeth is extremely important because of their high prevalence and number of complications after treatment [4, 6]. Focuses of periapical inflammation and bone destruction require new approaches to treatment . Therefore, it is important to develop new means and methods of treatment of chronic forms of periodontitis for biorevitalization and regeneration of tissues, damaged by inflammatory-destructive process, especially with an acquired wide tooth root apex [3, 5]. This led to the search for new means and improvement of methods of treatment that will stimulate biorevitalization and regeneration of tissues outside the apex . The aim of the study was to determine the dynamics of reparative processes in bone tissue under the influence of drugs and compositions for endodontic treatment based on the results of histological examination of bone tissue. The experiment was conducted on 80 white rats. To create a bone defect intervention was conducted under general anesthesia (0.5 ml of 4 % solution of sodium thiopental into the peritoneum). It was made a trapezoidal cut on the left side of the lower jaw of rats in the area between the incisor and right molar by scalpel incision and was separated the mucosa. It was created the bone defect diameter of 3 mm and a depth of 2.5 mm by spherical and fissure bur under constant irrigation with saline 0.9 %, which was filled with studied biomaterials or left with a blood clot covered by mucosa and stitched. All animals were divided into 4 research groups of 20 animals each: the first group – intact animals that served as a control; the second comparison group – animals on which it was created bone defect without biomaterial input; the third group – animals, on which to fill the defect it was used the mineral trioxide aggregate (MTA); fourth group – animals, whose defect was filled with the composition made from calcium hydroxyapatite and organic biorevitalizants (GA) (patent for utility model number 95974, Ukraine). From the experiment the rats were taken out on 14 and 30 day by their decapitation under anesthesia. Bone defect area of alveolar bone of the lower jaw of a rat was carved for further histological studies. Conducted studies indicate that the nature of morphological changes after the formation of defect using biomaterials and without them on 15 day varies in different degree of destruction of bone plates of subsequent lysis, accumulation of basophilic substance, infiltration of lymphoid elements of connective tissue structures. However, in histo-drugs of IV group of animals it was observed less pronounced signs of inflammation and destruction process of bone tissue, and a large number of active osteoblasts that synthesize bone matrix components, with the formation of thin bone beams show the regeneration of bone tissue. On 30 day in the second group of animals it was observed signs of inflammation, processes of destruction of bone plates and mild effects of bone tissue regeneration. In the third experimental group signs of inflammation and destruction process of bone plates were observed less pronounced and stimulation of the bone tissue regeneration was noticed. However, in the fourth group of animals while using the proposed compositions based on HA and organic biorevitalizants the signs of inflammatory responses were virtually absent, and processes of substitution reparations proceeded rapidly, as evidenced by the large number of newly formed blood vessels, the large number of active osteoblasts and synthesis of young bone beams. Therefore, to accelerate reparative processes of tissue outside the apex with chronic forms of periodontitis, especially with acquired wide tooth root apex, we can recommend using of the proposed osteotropic composition for the treatment of this disease.
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