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ECPB 2017, 78(2): 66–70
https://doi.org/10.25040/ecpb2017.02.066
Research articles

Influence of the Tiotriazolin on the Nitric Oxide System under the Conditions of the Development of experimental allergic Alveolitis

B. MELEKH
Abstract

Exogenous allergic alveolitis is a typical representative of interstitial diseases of the lungs, whose level is constantly increasing due to the rapid increase in the diversity of alerted animals.

A significant role was played in increasing the pathological processes of the development of oxidative stress and significant weakening of antioxidant defense. Some researchers have shown the key role of the medical strengthening of antioxidant defense in suppressing the course of exogenous allergic alveolitis. In addition to the significant role of oxidative stress in the pathogenesis of exogenous alergic alveolitis, an important role of the nitric oxide system in the respiratory system was identified. It is known that the activation of the nitric oxide system often leads to an increase in proinflammatory changes, presumably due to the increased formation of toxic peroxynitrite. In the literature available to us, there were no data related to the study of the effects of drugs with pronounced antioxidant properties on the processes of the system of nitric oxide under conditions of the development of allergic alveolitis. Experimental studies were performed on 45 guinea pigs in males with an average body weight of 280–320 g. The control group consisted of 15 intact males. The first experimental group consisted of 15 animals with experimental allergic alveolitis (EAA), which were withdrawn from the experiment on the 44th day after the start of the study. The second experimental group contained 15 animals with EAA that were withdrawn from the 44th day of the experiment from the start of the study and received tiotriazolin 50 mg / kg intramuscularly for 10 days starting at the 34th day. The content of stable metabolites of nitric oxide was determined based on the color reaction with the Grice reagent, the activity of iNOS and cNOS by the method of VV. Sumbaev, the concentration of L-arginine (L-Arg) – by the method of T.M. Aleinikov. The obtained results show significant changes in the system of nitric oxide, both in hemolysate and in hemogenate of the lungs. There was a significant increase in the concentration of stable metabolites of nitric oxide in hemolysates by 44 % at 37.89 % (p < 0.05) compared with the control group. However, in the second experimental group, after treatment with tiotriazolin, no significant safety was found in comparison with control. Also, iNOS activity in the first experimental group was significantly increased by 55.17 % (p < 0.05) compared with the control group. In the pulmonary tissue, changes in the nitric oxide system were manifested in a significant increase in iNOS activity by 36.36 % (p < 0.05) in the first experimental group compared to control and by 18.18 % (p < 0.05) in the second Experimental group compared to control. Additionally, iNOS activity was significantly reduced by 13.33 % (p < 0.05) in the second study group relative to the first experimental group. The results of this study showed a significant role of the nitric oxide system in the development of exogenous allergic alveolitis, both in the blood of animals and in the pulmonary tissue, mainly due to activation of the enzymatic link. Imbalance in the pulmonary tissue due to increased activity of inducible nitric oxide synthase and absence of reliable growth of stable metabolites of nitric oxide may be due to increased production of peroxynitrite due to the higher concentration of superoxidonium in the pulmonary tissue. The use of thiotriazoline in small doses (50 mg / kg) significantly inhibits the development of this pathology.

Keywords: allergic alveolitis, NO oxide, antioxidant system, thiothriazolin

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