Exogenous harmful substances entering the body are accompanied by the development of oxidative stress and accumulation of toxic products in various organs. Under these conditions, agents are used to remove toxic products of exo- and endogenous origin out of the body – enterosorbents are used.
Objective. Install the degree of endogenous intoxication and cytolytic syndrome in rats affected with sodium nitrite against the background of a 45-day intoxication tobacco smoke, and the efficacy of carboline enterosorbent under these conditions.
Materials and methods. The experiments have been conducted on three age groups of white male rats, that have been exposed to tobacco smoke for 45 days. One of group, 24 hours before the end of the experiment, has been administered with sodium nitrite at a dose of 45 mg/kg; the second – 72 hours before euthanasia. Two other groups, after the affection with both toxicants, have been intragastrically injected with enterosorbent carboline at a dose of 400 mg/kg for 30 days (from the 15th day of intoxication). After 45 days, the animals have been subjected to euthanasia under thiopental anesthesia. Іn blood serum of rats, the content of medium mass molecules has been determined, and the cytolysis of erythrocytic membranes has been evaluated by the percentage of their permeability, the degree of hepatocytes damage – by the activity of aminotransferases.
Results. After affection of the rats with tobacco smoke, in blood serum of the animals of all age groups an increase in the content of MSM in both fractions (SM1 and SM2) has been evidenced. The most susceptible to the action of both toxicants have been unborn rats, in which the content of SM1 in serum at the end of the experiment has increased by 4 times. After the introduction of carboline into the body of rats affected with both toxicants, in the last period of the study, this rate has decreased in the immature and senile rats in 2.6 times, in the mature – in 2.4 times. Similar changes have been evidenced in the SM2 fraction. At the simultaneous toxicity of animals with sodium nitrite and tobacco smoke, the percentage of permeability of erythrocyte membrane. Under the influence of carboline it has been decreased in blood of the rats of all age groups. The toxic effects of the studied xenobiotics have been manifested by hepatocyte cytolysis that has been proved by the increase of aminotransferases activity in serum after the affection and its decrease in liver. After administration of carboline into blood serum of the rats of all age groups the activity of AsAT has been decreased in 1.2 times. The enterosorbent has proved the highest efficacy in the group of senile rats. Introduction to the carboline-damaged organism has led to a decrease in the activity of ALT in serum. In the liver of rats, the activity of enzyme has been decreased after the affection, the use of carboline it has increased.
tobacco smoke, a large number of secondary endogenous toxins have been accumulated in the body and it has been accompanied by the development of cytolytic syndrome. This has been proved by the increase of erythrocytic index of intoxication and activity of aminotransferases in blood serum. The applied carboline has had a positive effect on the content of mediums molecular peptides, lowering them in serum, and has led to the normalization of aminotransferase activity in both serum and in the liver of affected animals.
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