online ISSN 2415-3176
print ISSN 1609-6371
logoExperimental and Clinical Physiology and Biochemistry
  • 8 of 12
ECPB 2018, 82(2): 56–63
Research articles

Examination of the Parameters of Antioxidant System and Energy Metabolism in the Rats that have Insulin Resistance Complicated by Iodine Deficit


The changes in the parameters of antioxidant system (AOS) and energy metabolism of rats that have insulin resistance (IR) complicated by iodine deficiency (ID) have been analyzed in the paper. 90 rats-females have been investigated. They have been divided into three experimental groups: animals with ID, IR and IR complicated with ID. The state of ID has been reproduced by keeping the rats on two-month iodine-deficient diet. In order to simulate IR, a 10 % solution of fructose have being added into the drinking water of animals during 8 weeks. The activity of catalase (K), ceruloplasmin (Cp), superoxide dismutase (SOD), glutathione peroxidase (GP), glutathione reductase (GR) and saturation of transferrin by iron (FTr) has been found in AOS of serum. Studying of energy metabolism has been based on the activity of succinate dehydrogenase (SDH), malate dehydrogenase (MDH) and lactate dehydrogenase (LDH) in blood serum.. It has been found that the development of ID has been accompanied by the changes of the AOS, primarily, the decreasing activity of GP (by 57.90 %) and GR (by 76.47 %) relatively to the initial indexes. Iodine deprivation has led to the changes of the system of cell energy supply, that has been manifested by a significant decrease of LDH activity.

Two straightened changes in the antiradical defense components have been found in the rats with IR. Thus, K activity has been lower by 66.73 %, while the GP activity has increased by 63.16 % comparely to the similar indexes in animals of the control group. The development of hyperglycemia has led to the disbalance of energy supply system. In particular, the activity of SDH and LDH has been decreased by 57.66 % and 97.58 % respectively in animals with IR, while the background of the activity of MDH has increased by 62.81 % relatively to the initial data. IR complicated by ID has led to the increasing of the activity of GP in 5,63 times, GR - in 4,50 times, on the contrary, the background of FTr has been decreased by 47,62 % in animals with iodine deprivation. In this case, the activity of SOD and CP in rats from the 3rd research group has been on 19.89 % and 46.96 % less than in in the conditions of isolated IR.

The changes in the system of energy metabolism of animals with endocrine pathology have been manifested by the suppression of serum dehydrogenases activity. In particular, the decrease of the activity of SDH (by 90.38 %), MDH (by 9.92%) and LDH (by 97.12 %) relatively to the control indexes, SDH (by 87.91 %) and LDH (by 45.93 %) relatively to the data about the rats with ID, SDH (by 71.45 %) and MDH (by 44.67 %) comparely to the rats with IR has been found. The predominant decrease of the activity of investigated enzymes in the energy balance can be regarded as a sign of the insufficient oxygen supply of the tissues and a malfunction of the biotransformation energy system that undergo more pronounced changes in the case of combined endocrine disorders.

Thus, the development of ID and IR has been accompanied by the changes of the activity of enzymes of antioxidant defense and macroenergy synthesis. The combined endocrine pathology has led to more severe changes of the studied parameters, that may indicate a violation of prooxidant- antioxidant homeostasis and cellular energy metabolism under the conditions of hyperglycemia against the background of ID. Violations of energy supply against the background of the suppression of antioxidant defense can be a pathogenic factor of diabetes mellitu disorders, the formation of which can potentiate ID.

Article recieved: 15.05.2018

Keywords: insulin resistance, iodine deficiency, antioxidant system, enzymes of energy metabolism

Full text: PDF (Ukr) 275K

  1. 1. Babenko GO. Vyznachennia mikroelementiv i metalofermentiv v klinichnykh laboratoriiakh. К.: Zdorovia;1968:137.
  2. 2. Bila AY, Krasnoselskyy MV, Gramatyuk SM. The state of bioenergy metabolism and antiox- idant enzymes in patients with breast cancer. Bulletin of Problems of Biology and Medicine. 2015;4(121):77-80.
  3. 3.Voronych-Semchenko NM, Guranych TV. Changes of processes of free radical oxidation of lipids and proteins, antioxidant defense in rats with hypofunction of thyroid gland on the background of iodine and copper deficit. Physiological Journal. 2014;60(4):30-9.
  4. 4. Ieshhenko ND, Prohorova MI. Metody biokhemicheskhikh issledovaniy: uchebnoe posobie. Izdatelstvo Leningradskogo Universiteta. 1982:272.
  5. 5. Zagayko AL, Briukhanova TO. Mechanisms of metformin influence on the nitrogen oxide system investigation at experimental insulin resistance in rats. Ukr Biofarm J. 2017;2(49):23-6.
  6. 6. Zamorskii II, Bukataru YS, Melnychuk SP. Screening of derivatives of 2-(benzoyilamino) (1-r-2-oxoindolin-3-ylidene) acetic acid under the conditions of acute hypobaric hypoxia. Scientific Journal ′′ScienceRise: Pharmaceutical Science′′. 2017;2(6):9-13.
  7. 7. Ivankiv YaI, Oleshchuk OM, Dacko TV, Fedoniuk LYa. Prooxidant-antioxidant homeostasis, carbohydrat e metabolism indicat ors and morphological changes in the liver after melatonine usage in experimental type 2 diabetes. Bul of Morphology. 2016;2(22):253-8.
  8. 8. Kikhtyak OP, Lishchuk OZ, Moskva KhA. Studying of the beneficial effect of insulin sensitizer on the carbohydrate and lipid metabolism in patients with diffuse toxic goitre and insulin resistance. Achievements of Clinical and Experimental Medicine. 2016;4:51-5.
  9. 9. Kopylchuk GP, Voloshcuk OM. NADH: Ubiqinone Reductase and Succinate Dehydrogenase activity in the liver of rats with Acetaminophen-induced toxic hepatitis on the background of alimentary protein deficiency. Ukr. Biochem. J. 2015;87(1):121-6.
  10. 10. Korolyuk MA, Ivanova LI, Mayorova IG. Metod opredelenia aktiv- nosti katalazy. Laboratornoe Delo. 1988;1:16-9.
  11. 11. Lykhatskyi P. Examination of indexes of the endogenous antioxidant system in rats affected with sodium nitrite on the background of tobacco intoxication. Science Rise: Biological Science. 2017;5(8):18- 23.
  12. 12. Lozinska LM, Semchychyn HM. Biological aspects of non-enzymatic glycosylation. Ukr Biochem J. 2012;84(5):16-37.
  13. 13. Pankiv VI. State of mineral density of bone tissue of women in postmenopausal period with primary hypothyroidism. Clinical & Experimental Pathology. 2016;1(55):104-10.
  14. 14. Furka OB, Ivanusa IB, Mykhalkiv MM, Klishch IM. Changes of some indices of antioxidant system in rats with type 2 diabetes mellitus and acetaminophen toxic lesions. Medical and clinical chemistry. 2017;19(1):25-30.
  15. 15. Chevari S, Chaba I, Sekey Y. Opredelenie antioksidantnykh parametrov krovi i ikh diagnosticheskoe znachenie. Laboratornoe delo. 1995;11:678-81.
  16. 16. Chuhai OO. Comparison of lipid peroxidation and activity of antioxidant enzymes in the mucosa of paradontium in different periods of formation of experimental pneu- monia. Achievements of Clinical and Experimental Medicine. 2016;4:125-30.
  17. 17. Shuprovych AA. Violation of uric acid metabolism in rats with experimental insulin resistant syndrome induced by fructose. Physiological Journal. 2011;57(1):72-81.
  18. 18. Berhane F, Fite A, Daboul N, Al-Janabi W, Msallaty Z, Caruso M. et al. Plasma lactate levels increase during hyperinsulinemic euglycemic clamp and oral glucose tolerance test. J. Diabetes Res. 2015;102054.
  19. 19. Chouchani ET, Kazak L, Spiegelman BM. Mitochondrial reactive oxygen species and adipose tissue thermogenesis: Bridging physiology and mechanisms. Journal of Biological Chemistry. 2017; 292(41):16810-6.
  20. 20. Dunn J, Grutchfield H, Gutekunst R. Two simple methods for measuring iodine in urine. Thyroid. 1993;3:119-23.
  21. 21. Hurrle S, Hsu W. The etiology of oxidative stress in insulin resistance. Biomedical Journal. 2017;40(5):257-62.
  22. 22. Pieme C, Tatangmo J, Simo G, Biapa СР, Ama Moor V, Moukette B et al. Relationship between hyperglycemia, antioxidant capacity and some enzymatic and non- enzymatic antioxidants in African patients with type 2 diabetes. BMC Res Notes; 2017;10:1-7.
  23. 23. Seung-Hwan L. Reactive oxygen species modulate immune cell effector function. J Immunol. 2017;198:328-37.

Програмування -