online ISSN 2415-3176
print ISSN 1609-6371
logoExperimental and Clinical Physiology and Biochemistry
  • 1 of 14
Up
ECPB 2018, 83(3): 5–17
https://doi.org/10.25040/ecpb2018.03.005
Research articles

Sexual Dimorphism of the Neuroendocrine-Immune Complex and its Reactions to Chronic Stress in Rats

I.L. POPOVYCH1, І.S. POLOVYNKO2, L.M. ZAJATS2, O.I. MELNYK3
Abstract

Introduction. If the sexual differences in endocrine parameters are well understood, then research of immune parameters in this aspect remains relevant.

Materials and methods. The experiment was performed on 50 males and 60 females of the Wistar line rats. Parameters of HRV, blood and daily urine levels of hormones and electrolytes as well as parameters of leukocytogram, immunocytogram, thymocytogram and splenocytogram were determined.

Results. It was found that the most significant sexual differences were observed in the morpho-functional parameters of the adrenal glands. Intact females had the highest androgenic, glucocorticoid and mineralocorticoid activity in particular, as well as parathyrin and calcitonin activity. The HRV-markers of sympathetic and vagal tones were not significantly different. In- stead, the plasma level of triiodothyronine in females was 85 % of males. Among the recorded immune indices, 11 of them were significantly higher in females. First of all, it was the content in the thymocytogram of lymphocytes and lymphoblasts, in the immunocytogram of blood natural killers and B-lymphocytes as well as fibroblasts, macrophages and microphages in splenocyto- gram. Instead, the 10 indicators of immunity in females were significantly lower. This was, first of all, the activity of phagocytosis of microphages and macrophages. The discrepancies between neuroendocrine-immune complexes of intact and stressed rats of both sexes were exhaustively explained by 38 parameters including 6 of thymus, 6 of spleen, 13 of blood and 13 of neuroen- docrine ones. The information contained in these metrics can be condensed in three recognition roots. Intact and stressed males were clearly delimited along the axis of the second root which reflected the post-stress decrease in the phagocytic activity of monocytes in the blood and the content of basophils and segmental neutrophils in it, on the one hand and the increase in the mass of thymus and the content of Hassall's cells in it, and the increase of the sympathetic tone and corticosterone - on the other hand. Post-stress changes of the listed parameters in general were insignificant in females. Significant differences between intact and stressed females were reflected along the third root axis which contains the information on stress induced lowering levels of parathyrin and thyroxin, the intensity of Staph aureus phagocytosis by microphages and bactericidal capacity of blood macrophages, its content of eosinophils as well as the mass of the spleen. Instead, post-stress changes in the listed parameters were generally insignificant in males.

Conclusions. The obtained data indicate the need to take into account the sexual diffe- rences in the reactions of the neuroendocrine-immune complex to stress when testing the stress limiting means.

Keywords: sexual difference, neuroendocrine-immune complex, stress, immunological parameters, immunogram, neuroendocrine indices

Full text: PDF (Eng) 877K

References
  1. 1. Baevskiy RM, Kirillov OI, Kletskin SZ. Mathematical Analysis of Changes in Heart Rate by Stress [in Russian]. Moskva: Nauka. 1984. 221 p.
  2.  2. Bazarnova MA. Cytology investigation punctates spleen. In: Guide to practical training in clinical laboratory diagnostics [in Russian]. Kyiv: Vyshcha shkola. 1988: 263-4.
  3.  3. Bianco C. Population of lymphocytes bearing a membrane receptor for antigen-antibody complex. J Exp Med. 1970; 134(4): 702-20. doi.org/10.1084/jem.132.4.702
  4. 4. Bilas VR, Popovych IL. Role of microflora and organic substances of water Naftussya in its modulating influence on neuroendocrine-immune complex and metabolism [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(1): 68-102.
  5. 5. Douglas SD, Quie PG. Investigation of Phagocytes in Disease. Churchil. 1981. 110 p.
  6. 6. Jondal M, Holm G, Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972; 136(2): 207-15. doi.org/10.1084/jem.136.2.207
  7. 7. Klecka WR. Discriminant Analysis [trans. from English in Russian] (Seventh Printing, 1986). In: Factor, Discriminant and Cluster Analysis. Moskva: Finansy i Statistika. 1989: 78-138.
  8. 8. Kozyavkina OV, Kozyavkina NV, Gozhenko OA, Gozhenko AI, Barylyak LG., Popovych IL. Bioactive Water Naftussya and Neuro-Endocrine- Immune Complex [in Ukrainian]. Kyiv: UNESCO-SOCIO. 2015. 349 p.
  9. 9. Lapovets' LYe, Lutsyk BD. Handbook of Laboratory Immunology [in Ukrainian]. Lviv. 2002. 173 p.
  10. 10. Limatibul S, Shore A, Dosch HM, Gelfand EW. Theophylline modulation of E-rosette formation: an indicator of T-cell maturation. Clin Exp Immunol. 1978; 33(3): 503-13.
  11. 11. Polovynko IS. Integrated quantitative estimation of neuro-endocrine manifestations of chronic stress in female rats. Experimental and Clinical Physiology and Biochemistry. 2017; 3(79): 5-10. doi.org/10.25040/ecpb2017.03.005
  12. 12. Polovynko IS, Popovych IL. Immunotropic traits in responses to chronic stress rats males [in Ukrainian]. In: VV Podvysotskiy XI reading. Bulletin Materials Science Conference (Odesa, 24-25 May, 2012). Odessa: Ukrainian Research Institute of Transport Medicine, 2012: 109-10.
  13. 13. Polovynko IS, Popovych IL. Variety immunotropic responses to chronic stress and neuroendocrine accompaniment of male rats [in Ukrainian]. In: Mater. V scientific-practical conference "Issues of pathology in conditions of extreme factors action on the body" (Ternopil, November 1-2, 2012). Achievements of Clinical and Experimental Medicine. 2012; 2(17): 200.
  14. 14. Polovynko IS, Zajats LM, Popovych IL. Neuroendocrine-immune relationships in males rats in conditions of chronic stress [in Ukrainian]. In: Mater. VI scientific-practical conference "Issues of pathology in conditions of extreme factors action on the body" (Ternopil, October 31-November 1, 2013). Achievements of Clinical and Experimental Medicine. 2013; 2(19): 274.
  15. 16. Polovynko IS, Zayats LM, Zukow W, Popovych IL. Neuro- endocrine-immune relationships by chronic stress at male rats. Journal of Health Sciences. 2013; 3(12): 365-74.
  16. 17. Polovynko IS, Zajats LM, Zukow W, Yanchij RI, Popovych IL. Quantitative evaluation of integrated neuro-endocrine and immune responses to chronic stress rats male. Journal of Education, Health and Sport. 2016; 6(8): 154-66.
  17. 18. Polovynko IS, Zukow W. Variety of immune responses to chronic stress in rats male. Journal of Education, Health and Sport. 2016; 6(12): 843-56.
  18. 19. Popovych IL. Functional interactions between neuroendocrine-immune complex in males rats [in Ukrainian]. Achievements of Clinical and Experimental Medicine. 2008; 2(9): 80-7.
  19. 20. Popovych IL. The concept of neuroendocrine-immune complex (Review) [in Russian]. Medical Hydrology and Rehabilitation. 2009; 7(3): 9-18.
  20. 21. Sternberg EM. Neural regulation of innate immunity: a coordinated nonspecific host response to pathogens. Nat Rev Immunol. 2006; 6(4): 318-28. 22. Thayer JF, Sternberg EM. Neural aspects of immunomodulation: Focus on the vagus nerve. Brain Behav Immun. 2010; 24(8): 1223-8.
  21. 23. Tracey KJ. Reflex control of immunity. Nat Rev Immunol. 2009; 9(6): 418-28. doi.org/10.1038/nri2566
  22. 24. Uchakin PN, Uchakina ON, Tobin BV, Ershov FI. Neuroendocrine immunomodulation [in Russian]. Vestnik Ross AMN. 2007; 9: 26-32.
  23. 25. Yushkovs'ka OG. Using information theory to study adaptive responses in the body athletes [in Ukrainian]. Medical Rehabilitation, Kurortology, Physiotherapy. 2001; 1(25): 40-3.
  24. 26. Zajats LM, Polovynko IS, Zukow W. Features neuro-endocrine support diversity of immune responses to chronic stress in male rats. Journal of Education, Health and Sport. 2017; 7(3): 97-105.
  25. 27. Zajats LM, Polovynko IS, Zukow W, Yanchij RI, Mysakovets' OG, Mel'nyk OI, Hrytsak YaL. Neuroendocrine-immune relatioships in rats females. Journal of Education, Health and Sport. 2017; 7(10): 59-78.


Програмування - Roman.im