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ECPB 2018, 83(3): 76–83
https://doi.org/10.25040/ecpb2018.03.076
Research articles

Comparative Characteristics of the Fatty Acid Composition of Phospholipids and Total Lipids in the Tissues of Myocardium, Liver and Blood Plasma of Rats under the Radiation Exposure with Previous Application of Hydrogen Sulfide Donor

I.M. KOVALCHUK
Abstract

Introduction. The preservation of the structural and functional status of cellular and subcellular membranes was largely ensured by the system of homeostasis of membrane lipids, one of the first ones undergoing changes in the action of small doses of radiation. The determination of FAC of lipids may be a criterion for monitoring the progress of post-radiation reaction and a method for assessing the effectiveness of correction.

Purpose. The purpose of our study was to compare changes in the FAC of phospholipids and total lipids of the tissues of the heart, liver and blood plasma under the influence of ionizing radiation with the previous application of hydrogen sulfide donor.

Materials and methods. Experimental studies were performed on sexually mature male rats. The irradiation of animals was performed in a single-fraction completely, absorbed a total dose – 2 Gy with the application of hydrogen sulfide donor in a dose of 7,4 mg/kg. The determination of the FAC of phospholipids of the heart muscle, liver and blood plasma was performed by a gas-liquid chromatography.

Results. The application of hydrogen sulfide donor NaHS in 30 min resulted in the increase in the level of omega-3 polyunsaturated fatty acids (PUFAs) in phospholipids and total lipids in the myocardium tissue comparing to the control one. The revealed nature of changes in the composition of PUFAs remained the same during the whole day after the introduction of NaHS. The effect of radiation after the first day caused a significant decrease in the levels of such omega-3 PUFAs in phospholipids and total lipids. The effect of radiation was manifested in the increase in the content of omega-6 acids, the decrease in the ratio of omega-3/omega-6 as compared to the control one. The similar nature of changes of the FAC in phospholipids and total lipids was observed in the liver tissue under the influence of investigated factors. One day after the exposure to radiation, there was a significant decrease in the level of omega-3 in phospholipids and total lipids, a probable growth in phospholipids and in total lipids as compared to the control group, the reduction in the ratio of omega-3/omega-6 PUFAs in phospholipids and total lipids. А significant increase in the ratio of omega-3/omega-6 was established in 30 minutes after the application of NaHS in the blood plasma. The level of omega-3 PUFAs of phospholipids decreased, the content of omega-6 PUFA increased one day after the radiation. Thus, the ratio of omega-3/ omega-6 PUFA decreased as compared to the control group. Prior to the radiation application of hydrogen sulfide donor leads to a partial restoration of the content of FAC in phospholipids and total lipids, the increase in the ratio of omega-3/omega-6 to the effect of ionizing radiation in all investigated tissues was observed.

Conclusions. Changes in the FAC of phospholipids and the absence of significant changes in FAC of total lipids have been detected in plasma. Under the conditions of the previous radiation action, the introduction of a hydrogen sulfide donor leads to a partial improvement of the profile of PUFAs in phospholipids and total lipids in the myocardium and liver and PUFAs in phospholipids of the blood plasma of experimental animals. These changes in PUFAs composition were a criterion for prognostically favorable conformational rearrangements due to the activation of H2S-ergic mechanisms during the development of adaptive processes to the action of small doses of radiation.

Article recieved: 05.09.2018

Keywords: omega-3 and omega-6 polyunsaturated fatty acids, myocardium, liver, blood plasma, hydrogen sulfide donor, ionizing radiation

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