The presence of interconnections between the parameters of the nervous, endocrine and immune systems within the framework of the triple complex is well known. However, data on the nature (synergism or antagonism) of the influence of the sympathetic and vagus link of the autonomic nervous system on the individual parameters of immunity are ambiguous. One of the approaches to solving this problem is to compare immunity parameters in individuals with different states of the autonomic nervous system. This is what determined the purpose of this study.

The experiment is at 60 female and 50 male white rats Wistar line weighing 210-320 g. Of these for 10 animals of each sex not subjected to any influences and others within 6-7 days were subjected to different stressing or adaptogenic manipulations. The day after the completion of manipulations in all rats determined parameters of heart rate variability and leukocytogram.

The next day, the animals were decapitated, for the purpose of collecting blood. The plasma levels of the hormones of adaptation were determined: corticosterone, triiodothyronine, thyroxine and testosterone; as well as electrolytes: calcium, phosphates, sodium and potassium for evaluation parathyrin, calcitonin and mineralocorticoid activities. On the state of the phagocytic function of microphages and macrophages judged by phagocytic index, microbial number and index of killing regarding museum culture Staphylococcus aureus. Among the parameters of immunogram determined the relative amount of blood population of T-cells, their theophylline resistant and sensitive subpopulations as well as the population of B-lymphocytes and Natural killers. From the spleen and thymus did smears for counting splenocytogram and thymocytogram. For the latter, as well as immunocytogram and leukocytogram we calculated entropy.

Three groups were formed according to the integral state of the autonomic nervous system evaluated by Baevskiy Stress-index. The eutonia was confirmed in 25 rats (14 males and 11 females). In 33 rats (12 males and 21 females) vagotonia was confirmed, whereas in 52 rats (24 males and 28 females) sympathicotonia was observed. Discriminant analysis detected 17 parameters, the totality of which three states of autonomous regulation differ significantly. In particular, in addition to the three HRV parameters, by definition, three endocrine parameters, two parameters of thymocytogram, five parameters of splenocytogram and three immune parameters of blood appeared to be characteristic.

Qualitatively different states of the autonomic nervous system of rats are accompanied by a characteristic constellation of endocrine and immune parameters, one set of which is subordinate to the opposite effects of the sympathetic and vagus link, whereas the other set contains parameters that are not significantly different in sympathicotonic and vagotonic animals and are characteristic of the eutonia.

Recieved: 25.03.2019

Keywords: heart rate variability, corticosterone, triiodothyronine, thyroxine, testosterone, phagocytosis, cellular and humoral immunity, female and male rats

Full text: PDF (Eng) 446K

1. 1. Baevskiy RM, Kirillov OI, Kletskin SZ. Mathematical Analysis of Changes in Heart Rate by Stress [in Russian]. Moskva: Nauka; 1984: 221 p.
2. 2. Bianco C. Population of lymphocytes bearing a membrane receptor for antigen-antibody complex. J Exp Med. 1970; 134(4): 702-20. doi.org/10.1084/jem.132.4.702
3. 3. Bilas VR, Popovych IL. Role of microflora and organic substances of water Naftussya in its modulating influence on neuroendocrine-immune complex and metabolism [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(1): 68-102.
4. 4. Goryachkovskiy АМ. Clinical Biochemistry [in Russian]. Odesa: Astroprint; 1998: 608 p.
5. 5. Hrytsak YL, Barylyak LG, Zukow W, Popovych IL. Cluster analysis of hormonal constel- lation at women and men with harmonious and disharmonious general adaptation reactions. Journal of Education, Health and Sport. 2016; 6(4): 141-50.
6. 6. Hrytsak YL, Mysakovets' OG, Mel'nyk OI, Barylyak LG. Features of immunity by various constellations of principal adaptation hormones and autonomous regulation in rats. Experimental and Clinical Physiology and Biochemistry. 2017; 4(80): 13-23. doi.org/10.25040/ecpb2017.04.013
7. 7. Instructions for application for recruitment reagents for ELISA investigations hormones in the blood of humans [in Russian]. St. Petersburg: JSC «Alkor Bio»; 2000: 21 p.
8. 8. Jondal M, Holm G, Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972; 136(2): 207-15. doi.org/10.1084/jem.136.2.207
9. 9. Khaitov RM. Physiology of immune system [in Russian]. Moskva: VINITI RAN; 2005: 428 p.
10. 10. Klecka WR. Discriminant Analysis [trans. from English in Russian] (Seventh Print- ing, 1986). In: Factor, Discriminant and Cluster Analysis. Moskva: Finansy i Statistika. 1989: 78-138.
11. 11. Kozyavkina OV. Post-stress changes in neuroendocrine status and metabolism in rats with different types of initial vegetative homeostasis induced by bioactive water Naftusysa [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(1): 42-50.
12. 12. Kozyavkina OV. State of post-stress parameters of vegetative homeostasis and endocrine, metabolic and immune status as well as the relationship between them in rats with alternative types of precursor vegetative homeostasis induced by bioactive water Naftussya [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(2): 40-56.
13. 13. Kozyavkina OV. Vegetotropic effects of bioactive water Naftussya in male rats and their endocrine, electrolyte and immune accompaniments [in Ukrainian]. Medical Hydrology and Rehabilitation. 2012; 10(3): 65-92.
14. 14. Kozyavkina OV, Barylyak LG. Ambivalent vegetotropic effects of bioactive water Naf- tussya and opportunity of their forecasting at rats. Medical Hydrology and Rehabilitation. 2008; 6(3): 123-7.
15. 15. Kozyavkina OV, Kozyavkina NV, Gozhenko OA, Gozhenko AI, Barylyak LG, Popovych IL. Bioactive Water Naftussya and Neuro-Endocrine-Immune Complex [in Ukrainian]. Kyiv: UNESCO-SOCIO. 2015. 349 p.
16. 16. Lapovets' LYe, Lutsyk BD. Handbook of Laboratory Immunology [in Ukrainian]. Lviv; 2002: 173 p.
17. 17. Limatibul S, Shore A, Dosch HM, Gelfand EW. Theophylline modulation of E-rosette formation: an indicator of T-cell maturation. Clin Exp Immunol. 1978; 33(3): 503-513.
18. 18. Popovych IL. Functional interactions between neuroendocrine-immune complex in males rats [in Ukrainian]. Achievements of Clinical and Experimental Medicine. 2008; 2(9): 80-87.
19. 19. Popovych IL. Influence of the course on the use of bioactive water Naftussya on vegetative regulation in rats in basal and post-stress periods [in Ukrainian]. Medical Hydrology and Re- habilitation. 2008; 6(2): 79-83.
20. 20. Popovych IL. The concept of neuroendocrine-immune complex (Review) [in Russian]. Medical Hydrology and Rehabilitation. 2009; 7(3): 9-18.
21. 21. Popоvych IL. The factor and canonical analysis parameters of neuro-endocrine-im- mune complex, metabolism and erosive-ulcerose injuries of mucous stomach at rats in conditions of acute water іmmersing stress [in Ukrainian]. Medical Hydrology and Reha- bilitation. 2007; 5(2): 68-80.
22. 22. Popovych IL, Polovynko IS, Zajats LM, Mel'nyk OI. Sexual dimorphism of the neuroendocrine-immune complex and its reactions on chronic stress at rats. Experimental and Clinical Physiology and Biochemistry. 2018; 3(83): 5-17. doi.org/10.25040/ecpb2018.03.005
23. 23. Popovych IL, Struk ZD, Vis'tak-Markevych HI, Duda NB, Korda MM. Sexual dimor- phism of reactions of the neuroendocrine-immune complex and metabolism to adaptogenic bal- neotherapy in individuals with dysadaptosis. In: Proceedings XI Scientific Conference «Issues of pathology in conditions of extreme factors action on the body» (Ternopil', 4-5 October 2018). Ternopil'. 2018: 49-50.
24. 24. Popovych IL, Vis'tak HI, Gumega MD, Ruzhylo SV. Vegetotropic Effects of Bioactive Water Naftussya and their Endocrine-Immune, Metabolic and Hemodynamic Accompaniments [in Ukrainian]. Kyiv: UNESCO-SOCIO; 2014: 163 p.
25. 25. Sydoruk NO, Gozhenko AI, Zukow W. Modulating effects of bioactive water Naftussya from layers Truskavets' and Pomyarky on neuro-endocrine-immune complex and metabolism at rats exposed to acute stress. Journal of Education, Health and Sport. 2016; 6(11): 715-30.
26. 26. Vis'tak HI, Popovych IL. Vegetotropic effects of bioactive water Naftussya and their endocrine and immune support in female rats [in Ukrainian]. Medical Hydrology and Rehabi- litation. 2011; 9(2): 39-57.