online ISSN 2415-3176
print ISSN 1609-6371
logoExperimental and Clinical Physiology and Biochemistry
  • 6 of 13
ECPB 2019, 85(1): 38–43
Research articles

Adenilate System of Rat Erythrocyts with a Hyperactive Urinary Bladder for its Correction by the Compositions of Testosterone/Estradiol with Quercetin


According to ICS, hyperactive urinary bladder syndrome is among the 10 most common diseases, with amount of 100 million people in the world whom are ill, and is ahead in the frequency of diseases of diabetes mellitus, stomach ulcer and duodenal ulcer By expenditure, this disease is in the fifth place after arthritis, urinary incontinence and osteoporosis. Costs of GAMP are comparable to the cost of treatment for asthma and osteoporosis. Treatment of GAMP is necessary in all cases where the available symptoms cause a decrease in the quality of life of the patient. Taking into account the significant negative influence of GAMP on the quality of life of patients, it is possible to appoint therapeutic measures before the completion of all diagnostic procedures.

Since the exact causes of development and pathogenesis of GAMP are still not fully established, treatment of these patients remains largely symptomatic. Preparations of choice in treating GAMP are M-cholinoblockers. The use of M-cholin blockers in GAMP can reduce the frequency of urination, reduce the number of episodes of incontinence, increase the functional capacity of the bladder. There is also information on the promising use of flavonoids in the treatment of GAMP, including some methods are protected by the patent for the invention.

Taking into account not fully learned reason of development and pathogenesis of GAMP, it is promising to use combined drugs therapy with multi-directional mechanisms of action. The purpose of our work was to investigate the qualitative and quantitative indices of the adenylate system of erythrocytes of rats with hyperactive urinary bladder in its correction with testosterone / estradiol compositions with quercetin.

The influence of sex hormones (testosterone, estradiol) composition with quercetin on the qualitative and quantitative compound of adenile nucleotides of erythrocytes of rats with medicamentally produced, by our own methods, syndrome of hyperactive urinary bladder (HAUB) was studied.

It was defined that the HAUB course in experimental animals is accompanied by a probable decrease in the content of adenosine-triphosphate (ATP) to 14.69 % from the total number of adenile nucleotides against 58.6 % in intact with simultaneous increase of adenosine-diphosphate (ADP) to 74.65 % against 14.09 %, respectively. It was established that the course of GASM in experimental animals was accompanied by a significant imbalance of the adenylate system of erythrocytes, due to a decrease in the content of ATP and an increase in the content of ADP in erythrocytes, indicating the development of violations of biosynthetic processes The objective confirmation of the synergistic effect of sex hormones and bioflavonoids in the correction of functional disorders in cases of HAUB by increasing the compensatory mechanisms of cellular energy conservation has been received.

It has been substantiated that in the mechanism of development of HAUB significant importance belongs to the imbalance of the adenilate system of erythrocytes.

Recieved: 21.02.2019

Keywords: energetic profile, hyperactive urinary bladder, composition, testosterone, estradiol, quercetin

Full text: PDF (Ukr) 652K

  1. 1. Astapenko AV, Likhachev SA, Zabrodets GV. Neurogenic bladder: pathogenesis, classification, clinic, diagnosis, treatment. Belarusian honey journals 2002;2:4-6).
  2. 2. Michael J Kennelly, William B De Voe. Overactive Bladder: Pharmacologic Treatments in the Neurogenic Population. Rev Urol. 2008;3(10):182-91.
  3. 3. Henry Lai H, Teimothy B Boon, Rodney A Appell. Selecting a Medical Therapy for Overactive Bladder. Rev Urol. 2002;4(4):28-37.
  4. 4. Juan YS, Chuang SM, Lee YL, Long CY, Wu TH, Chang WC et al. Green tea catechins decrease oxidative stress in surgical menopause-induced overactive bladder in a rat model. BJU international. 2012;110:6b.
  5. 5. Yaw-Bin Huang, Ming-Wei Lin, Yun Chao, Chi-Te Huang, Yi-Hung Tsai, Pao-Chu Wu. Anti-oxidant activity and attenuation of bladder hyperactivity by the flavonoid compound kaempferol. International J Urology. 2014;21(1):94-8.
  6. 6. Fürer K, Eberli D, Betschart C, Brenneisen R, De Mieri M, Hamburger M, von Mandach U. Inhibition of porcine detrusor contractility by the flavonoid fraction of Bryophyllum pinnatum a potential phytotherapeutic drug for the treatment of the overactive bladder syndrome. Phytomedicine. 2015;22(1):158-64.
  7. 7. Seipel Tracey Anne. Herbal compositions for the prevention or treatment of urinary incontinence and overactive bladder. U.S. Patent No. 7/378.115. 27 May 2008.
  8. 8. Rodriguez Al. Quercetin-containing composition, methods of making, and methods of using. U.S. Patent Application No. 11/497.318.
  9. 9. Derzhavin VM, Vishnevsky EL, Gusev BS. Experimental study of inhibited neurogenic urinary tummy. Urol. and nephrol. 1977;4:32).
  10. 10. Atkinson DE. The energy charge of the adenylate pool as a regulatory parameter. Biochemistry. 1966;7:4030-4.
  11. 11. Dudzinska W1, Lubkowska A, Dolegowska B, Safranow K, Jakubowska K. Adenine, guanine and pyridine nucleotides in blood during physical exercise and restitution in healthy subjects. Eur J Appl Physiol. 2010;110:1155-62.
  12. 12. Morozov SL. Modern ideas about neurogenic dysfunction of the bladder. Russian Bulletin of Perinatology and Pediatrics. 2013;58:4).

Програмування -