We applied the method of infrared spectroscopy using the author’s technique blood smears (unstained, non-fixed) patients with diagnoses: breast cancer, fibroadenomatosis and practically healthy. Our research is based on an analysis of the spectral characteristics of blood in the Amide 1 band (1600 ... 1700 cm-1) where the spectrum is formed mainly due to the protein components of the blood, the main part is albumin. That is why the changes of the spectral indices reflect the conformational changes of albumin, changes the state of its liganding. Blood smears on a glass slide were dried for 40 minutes in termostat at t = 35º С. The measurements were issued on an infrared spectrophotometer Specord M 80 equipped with a reflection additional. For objectify of our data we are looking for possible options for supplement the quality description of spectrograms with quantitative indicators. With these indicators the infrared spectra could be objectively compared. During spectral studies of thin blood films from the entire infrared range available for study (4000 ... 200 cm-1), we selected a section (1672 ... 1658 cm-1).
A quantitative estimate of a portion of the spectrum was performed by calculating of the area under the graph of the modulus of the second derivative using the author’s computer program. Statistical processing of the results was performed by methods of variation statistics.
Specified differences in the spectral indices of healthy and patients with breast cancer were revealed and patients with breast cancer had the spectral indices of native smears significantly lower. Treatment of blood smears with a solution of calcium chloride alters the absorption. After the treatment of blood smears with calcium chloride, changes in absorption were different and significant in the groups of practically healthy and patients with breast cancer.
Practically healthy patients with a diagnosis of fibroadenomatosis the spectral indices of blood smears after treatment with calcium chloride showed a tendency to decrease, and patients with cancer – significantly increased, reaching values of the indicator of native blood smears of practically healthy people. As control and more thoroughly overview of the applying of the diagnostic capabilities of spectroscopy, we provided T-analysis of the results.
Multidirectionality of changes in absorption under the influence of a solution of calcium chloride for group “healthy” for patients with breast cancer and patients diagnosed with fbroadenomatosis can significantly complement the procedure of primary and differential diagnosis.
This approach for differential diagnosis is characterized by reliability, expressivity and high throughput.
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