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ECPB 2019, 86(2): 66–71
Research articles

Assessment of Dental Status in Rats with Experimental Hyperhomocysteinemia and its Correction with Choline and Vitamin D


Introduction. The results of clinical and anthropometric studies indicate the increase in the intensity of caries under the influence of HHC, as well as the occurrence of destructive changes in the alveolar process of the jaws of experimental rats in comparison with the healthy control group. The correction of HHC is possible by means of choline and vitamin D. The possible connection of the described dental disorders with changes in metabolic processes in HHC is discussed in this article. For many years, the physiological role of vitamins, vitamin D in particular, its importance for the functioning of the oral cavity has been studied. Another object of modern scientific research is homocysteine (HC), as an important physiological factor and predictor of many pathological conditions for hyperchomocysteinemia (HHC). A special attention is drawn to the links of the HHC with processes related to the dental pathology. The purpose of this study was to evaluate the dental status of female rats at experimental HHC and its correction with choline and vitamin D. Materials and methods. Animals were divided into 4 groups. Group 1 – the intact control one (n = 10), group 2 - animals in which thiolactone HHCs were modeled (n = 10), in 3 groups of animals, methionine HHCs (n = 10) were replicated, and in group 4, the simulation of thiolactone HHC was combined with its correction of choline and vitamin D (n = 10). The experiment lasted 28 days. In the process of research the visual estimation of the state of mucous membrane of oral cavity was conducted in vivo for the determination of presence and character of inflammatory process. The intensity of caries development was estimated by the method of counting the amount of carious teeth. Results. The presence of the chronic inflammatory process, of a non-intensive nature, was recorded in the area of gums of lateral teeth only in animals of group 2 with thiolactone HHC, and there were not so many cases, only at the end of the experiment. On the mucous membranes of the cheeks and lateral regions of the tongue in rats of the 2 group there were isolated ulcers of a small size, surrounded by inflammatory infiltrates of acute nature. The pathological mobility and periodontal pockets were not determined. In the area of incisors, not any pathological abnormalities were present. The largest degree of atrophy of a compact plate of the alveola was recorded in the 2nd group of animals (thiolactone HHC). The inclusion of choline and vitamin D in the diet of group 4 of rats effectively inhibits the resorption of alveoli as compared with group 2. Both of these indicators of the intensity of the carious process in the 2 and 3 groups of rats are significantly different from the control group and are practically the same. In the 4th group of animals, the number of carious teeth was a bit different from the similar indicators in group 2. Conclusions. HHC, regardless of the modeling technique, promotes the emergence of a moderate isolated lesion of a periodontal disease, which is remotely reminiscent of the clinical picture of the beginning of the development of generalized periodontitis. The correction of thiolactone-induced HHC by means of choline and vitamin D slows the processes of alveolar bone destruction. The presence of a small number of secondary lesions in various parts of the oral mucosal membrane is, in our opinion, symptomatic manifestations in animals of some somatic pathology induced by the imbalance of homocysteine and vitamin D. HHC also induces the process of demineralization of teeth enamel, and the suggested method of correction of HHC with the help of choline and vitamin D effectively inhibits the process of caries in fissures of indigenous teeth in rats of the experimental groups.

Recieved: 01.04.2019

Keywords: experimental hyperhomocysteinemia, choline, vitamin D, dental status

Full text: PDF (Eng) 445K

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