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ECPB 2019, 87(3): 37–43
Research articles

Effect of Experimental Hypergomocysteinemia and its Correction by Choline and Vitamin D on the Reproductive Function and Dental Status of Rates of Rats


Introduction. Hyperhomocysteinemia (HHC) appears when the level of homocystein in plasma is bigger than standart index. But the problems is that literature has no mention how to prevent to treat HHC using vitamin D or choline.

The purpose of this study was to evaluate the reproductive function and dental status of female rats in methionine-induced HHC and its correction with choline and vitamin D. Materials and methods. This experiment is divided into 4 stages: the reproduction of the model HHC and its correction with choline and vitamin D, fertilization of females, pregnancy, postpartum period.

In the first stage of the experiment we used 45 females weighing 200–220 g, divided into 3 groups, 15 individuals each. Animals of group 1 received basic starch-casein diet. In animals of groups 2 and 3, the methionine HHC model was reproduced for 28 days, and in the 3 – HHC model, choline and vitamin D were subjected to correction. In the course of the experiment, the following indicators were recorded: the body weight of the females, the duration of pregnancy, the number of newborns and their sex, the body weight of the newborn rats, assessment of the condition of various sections of the oral mucosa and determining the presence of pathological pockets and mobility of the teeth, carried out in vivo and determining the state of the alveolar bone and the intensity of the carious process – in vitro.

Results. Statistically, the indicators of body weight in all groups did not differ from each other. The gestation period in the 2 group of animals was shorter and significantly different from the indicators of the 1 and 3 groups, in which the duration of pregnancy was almost the same. The number and average body weight of the newborns in the group of females with methionine- induced HHC was significantly lower than in the intact control group and the 3 group. In general, the condition of periodontal tissues of rats of group 2 remotely corresponds to the clinical picture of generalized periodontitis of initial-1 severity in humans. The highest degree of atrophy of the compact plate of the alveoli was recorded in the second group of animals (methionine HHC), which was differed from the indicators of groups 1 and 3 by 19% and 14%, accordingly. In the 3 group of rats almost the same degree of alveolar resorption was determined in comparison with physiological atrophy in the control group of animals.

Conclusions. 1. Methionine HHC causes a violation of the process of pregnancy, which is manifested in reducing the duration of pregnancy, reducing the number of newborns, their sex (female) and body weight. 2. With prolonged unadjusted methionine HHC clinical picture of the condition of periodontal tissues of female rats corresponds to generalized periodontitis of the initial – I degree of severity. 3. The experimental methionine HHC enhances the processes of demineralization of tooth enamel. 4. HHC correction with choline and vitamin D effectively prevents inflammatory-destructive changes in periodontal tissues and in generally reduces the activity of the caries process.

In the long terms it is advisable to study the effect of prolonged methionine HHC and the violation of vitamin D status on blood biochemical parameters in female rats and their off spring.

Received: 27.08.2019

Keywords: experimental hyperhomocysteinemia, reproductive function, choline, vitamin D, dental status

Full text: PDF (Ukr) 439K

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