online ISSN 2415-3176
print ISSN 1609-6371
logoExperimental and Clinical Physiology and Biochemistry
  • 3 of 9
ECPB 2019, 88(4): 22–28
Research articles

The Biochemical Parameters of Blood and Homogenate of the Alveolar Bone of Female Rats at Long-term Experimental Methionine Hyperhomocysteinemia and its Correction with Choline and Vitamin D


Introduction. The mechanisms of pathogenetic action of hyperhomocysteinemia are known to be mainly associated with oxidative stress, impaired methylation, antioxidant protection etc. It has been experimentally and clinically proved that increases in serum HC also adversely affect calcium-phosphorus metabolism and bone mineral density. Similar disorders of bone mineralization are caused by deficiency of vitamin D. The above-mentioned changes in metabolism impact on the dental status of the body at different stages of life. Purpose of the research – To study the biochemical parameters of blood of female rats in methionine-induced HHC and its correction with choline and vitamin D for a long time Materials and methods The study was conducted on 30 adult female rats, divided into 3 groups: group 1 – intact control, group 2 – animals with methionine HНС and group 3 – animals which have HНС adjusted by choline and vitamin D. Adult females, including pregnancy, were 86-97 days for intact animals (group 1), 90–96 days for rats 2 groups and 90-97 days for 3 groups of females. At the end of the experiment, females were removed from the experiment under thiopental anesthesia and blood was collected from the heart and alveolar shoots in the mandibular area for biochemical studies performed in blood serum and bone homogenates. In the serum and in the alveolar bone homogenate of the experimental animals, the concentration of total protein, malondialdehyde, reduced glutathione, phospholipids and their fractional composition, total and ionized calcium and phosphate content, alkaline acid phosphatase, alpha phosphate activity, alkaline phosphatase activity, and alpha phosphate activity were determined, content of homocysteine and 25-hydroxyvitamin D (25-OH D). Results. Discussion The results obtained confirm the existing literature data according , that HНС and vitamin D deficiency in the body break calcium-phosphorus metabolism, which is actively reflected in the functioning of bone tissue. This negative effect causes structural changes in the periodontal bone tissue. The data of this study confirm our previous results and prove that HНС shows a significant decrease in the concentration in the blood of vitamin D, calcium and phosphates. The enrichment of the diet with choline and vitamin D effectively prevents the development of HНС and its characteristic changes in metabolism concerning oxidative stress, antioxidant protection, methylation, vitamin D status and phosphorus-calcium metabolism. Conclusions and prospects of further developments 1. Our experimental study showed, that during prolonged methionine HНС in female rats of the postpartum period appear signs of oxidative stress, inhibiting the processes of antioxidant protection and methylation. There is also a decrease in blood levels of vitamin D, calcium and phosphates, changes in the activity of alkaline and acidic phosphatases in blood elastase and alveolar bone homogenate. 2. The enrichment of the diet with choline and vitamin D prevents nearly all these biochemical abnormalities characteristic of HНС and prevents changes in the characteristics that characterize the destructive process in the solid periodontal tissues of experimental animals. 3. In general, the biochemical changes found in postpartum female rats are almost identical to those observed in non-pregnant animals. The prospect of the further development is to study the effects of long-term effects of hyperhomocysteinemia on the female rats on the biochemical processes in the blood and the dental status of their progeny, in the early postnatal period of life.

Received: 30.10.2019

Keywords: biochemical studies, experimental hyperhomocysteinemia, choline, vitamin D, female rats

Full text: PDF (Ukr) 412K

  1. 1. Kostyuchenko BI. Hyperhomocysteinemia: clinical significance, age characteristics, diagnosis and correction. Clinical gerontology. 2007;13(4):32-40.
  2. 2. Pentuk OO, Lutsyuk MB, Andrushko II, Postovitenko KP. Metabolism of homocysteine and its role in pathology. Ukr. biochem. 2003;75(1):5-17.
  3. 3. Owczarek D, Cibor D, Sałapa K, Jurczyszyn A, Mach T. Homocysteine in patients with inflammatory bowel diseases. Przegl. Lek. 2014;71(4):189-92.
  4. 4. Firsova IV, Mokrova EA, Zavodovsky BV, Makedonova YuА. Vitamin D and its role in the development of dental diseases (review article). Modern problems of science and education. 2014;6:34-9.
  5. 5. Ferlazzo G, Fries W. Vitamin D and inflammatory bowel disease. Biomed. Res. Int. 2015.
  6. 6. Gatti D, Idolazzi L, Fassio A. Vitamin D: not just bone, but also immunity. Minerva Med. 2016;107(6):452-60. 
  7. 7. Bezushko EV, Ugrin MM, Popovich ZB. Disorders of development and formation of teeth. Lviv. GalDent; 2007. 72 р.
  8. 8. Kutelmakh OI. Influence of experimental hyperhomocysteinemia and its correction with choline and vitamin D on biochemical parameters of blood of female rats. Experimental and Clinical Physiology and Biochemistry. 2019;3(87):37-43).
  9. 9. Voskresensky ON, Tkachenko EC, Chumakova YuH. Preclinical study of the means of prevention and treatment of periodontitis (periodontal protectors). Method. recommendations. Kiev. Gos. farm. center 2002. 16 р.
  10. 10. Menshikov VV. Laboratory research methods (reference). M. Medicine. 1987. 365 р.
  11. 11. Wagner VK, Putilin VM, Kharabuga GG. Methods and results of the study of isoenzymes (intestinal and hepatic fractions) of serum alkaline phosphatase in acute surgical diseases of abdominal organs. Question med. сhemistry. 1981;27(6):752-5.
  12. 12. Viser РL, Blout ER. The use of p-nitrophenyl - N - tert - butyl - oxycarbonyl - α - alaninate as substrate elastase. Biochem. Biophys. Acta. 1972;268(1):257-60.
  13. 13. Kozhemyakin YM, Khromov OS, Filonenko MA, Sayretdinova GA. Scientific and practical recommendation sfor the maintenance of laboratory animals and work with them. Kyiv; 2002. 155 р.
  14. 14. Kutelmakh OI, Lutsyuk MB, Lesik RB, Kutelmakh YuO, Popova OI. Influence of experimental hyperhomocysteinemia and its correction with choline and vitamin D on biochemical parameters of blood of female rats. Experimental and Clinical Physiology and Biochemistry. 2019;1(85):72-7).
  15. 15. Lee HJ, Je DI, Won SJ, Paik DI, Bae KH. Association between vitamin D deficiency and periodontal status in current smokers. Dent. Oral. Epidemiol. 2015;43(5):471-8.
  16. 16. Joseph R, Nath S, Joseraj M. Elevated plasma homocysteine levels in chronic periodontitis. Periodontol. 2011;82(3):439-44.

Програмування -