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ECPB 2020, 90(2): 14–20
Research articles

Influence of whole body vibration on structural properties of bone in conditions of obesity and limited mobility


In this study we assessed the impact of high-frequency whole body vibration with acceleration of 0,3 g on the structure of the femur in in conditions of obesity and limited mobility. It is known that mechanical loads stimulate bone remodeling by reducing the apoptosis of osteoblasts and osteocytes, increasing their proliferation and differentiation. Reducing the functional load inhibits osteogenesis and activates bone resorption. Aim. To study the effect of nonphysiological whole body vibration (acceleration 0,3 g) on bone remodeling and the condition of bone nanocomposite in conditions of obesity and limited mobility Materials and methods. The experimental study was performed on 54 male Wistar rats weighing 180-200 g in accordance with bioethical principles. Experimental rats were divided into 3 groups of 18 individuals: control – standard vivarium conditions, I experimental group – high-calorie diet and sedentary lifestyle, II experimental group – high-calorie diet and sedentary lifestyle + whole body vibration. Obesity model realized using high-calorie diet C 11024, (Research Diets, New Brunswick, NJ); and limited mobility – using cages with partitions that limit the mobility of rats. Vertical vibration oscillations were simulated using a vibrating table with a frequency of 50 Hz, 0,3 g. X-ray diffraction analysis of the prepared bone powder was used to characterize the crystalline organization of bone nanocomposites. The mineral mass of the femur was determined quantitatively by atomic adsorption analysis. Results. Using this experimental model, we proved that the mineral mass of the femur decreases from the 8th week. The decrease in the relative amount of crystalline phase lasted throughout the experiment, up to the 24th week of the study (p <0.05), and was not accompanied by violations of the nanostructure of the crystal lattice of hydroxyapatite. These structural changes were accompanied by a change in the quantitative indicators of calcium in the fragments of the femur. Obese and sedentary rats had lower bone mineral mass (p <0.05) compared to animals fed a normal diet and additionally affected by whole body vibration. Mechanical stimuli improved the structural and functional condition of the bones and prevented the accumulation of adipose tissue, as evidenced by changes in the weight of experimental rats. Conclusions. Whole body vibration with acceleration of 0,3 g, has a positive effect on body weight, has an antiresorptive effect, prevents bone loss during obesity, and did not destabilize the crystal lattice of the bone mineral composites at the end of the experiment. Therefore, exercises are an effective method to improve bone condition and should be used as an adjunct to pharmacological therapy for osteoporosis.

/ Added: 18.05.2020

Keywords: bone remodeling, bone mineral density, osteoporosis, X-ray diffract

Full text: PDF (Ukr) 495K

  1. 1. Smith KB, Smith MS. Obesity statistics. Primary care: clinics in office practice. 2016; 43(1): 121-35.
  2. 2. Reid IR, Ames RU, Evans MC, Sharpe S, Gamble G et al. Determinants of total body and regional bone mineral density in normal postmenopausal women--a key role for fat mass. The Journal of Clinical Endocrinology & Metabolism. 1992; 75(1): 45-51.
  3. 3. Villareal DT, Apovian CM, Kushner RF, Klein S. Obesity in older adults: technical review and position statement of the American Society for Nutrition and NAASO, The Obesity Society. Obesity research. 2005; 13(11): 1849-63.
  4. 4. Kostyshyn NM, Grzegotsky MR, Servetnyk MI. Assessment of structural and functional condition of rats bone tissue under the influence of various parameters of vibration. Current Issues in Pharmacy and Medical Sciences, 2018; 31(3), 148-53.
  5. 5. Lam TP, Ng BKW, Cheung LWH, Lee KM, Qin L., Cheng JCY. Effect of whole body vibration (WBV) therapy on bone density and bone quality in osteopenic girls with adolescent id- iopathic scoliosis: a randomized, controlled trial. Osteoporosis international. 2013; 24(5): 1623-36.
  6. 6. Pang MY, Lau RW, Yip SP. The effects of whole-body vibration therapy on bone turn- over, muscle strength, motor function, and spasticity in chronic stroke: a randomized controlled trial. European journal of physical and rehabilitation medicine. 2013; 49(4): 439-50.
  7. 7. Cao J. Effects of obesity on bone metabolism. Journal of orthopaedic surgery and re- search. 2011;6.1:30.
  8. 8. Villareal DT, Apovian CM, Kushner RF, Klein S. Obesity in older adults: technical review and position statement of the American Society for Nutrition and NAASO, The Obesity Society. Obesity research. 2005;13(11):1849-63.
  9. 9. McGee-Lawrence ME, Wenger KH, Misra S, Davis CL, Pollock NK, Elsalanty M et al. Whole-body vibration mimics the metabolic effects of exercise in male leptin receptor-deficient mice. Endocrinology. 2017;158(5):1160-71.
  10. 10. Huang CC, Tseng TL, Huang WC, Chung YH, Chuang HL, Wu JH. Whole-body vibration training effect on physical performance and obesity in mice. International journal of medical sciences. 2014;11(12):1218.
  11. 11. Bellia A, Salli M, Lombardo M, D'Adamo M, Guglielmi V, Tirabasso C et al. Effects of whole body vibration plus diet on insulin-resistance in middle-aged obese subjects. International journal of sports medicine. 2014;35(06);511-6.
  12. 12. Cao J, Sun L, Gao H. Diet induced obesity alters bone remodeling leading to de- creased femoral trabecular bone mass in mice. Annals of the New York Academy of Sciences. 2010;1192(1):292-7.
  13. 13. Maddalozzo GF, Iwaniec UT, Turner RT, Rosen CJ, Widrick JJ. Whole-body vibration slows the acquisition of fat in mature female rats. International journal of obesity. 2008;32(9):1348.
  14. 14. Minematsu A, Nishii Y, Imagita H, Sakata S. Whole body vibration at low-frequency can increase trabecular thickness and width in adult rats. Journal of musculoskeletal & neuronal interactionsю 2019;19(2):169. http
  15. 15. Rubin C, Turner AS, Bain S, Mallinckrodt C, McLeod K. Low mechanical signals strengthen long bones. Nature, 2001;412(6847):603-4.
  16. 16. Physiological mechanisms of bone tissue remodeling under the impact of different vibration conditions (experimental study). ref. dis. PhD: 14.03.03/Kostyshyn Nazar Mikhailovich. Lviv, 2018. 20 p. [in ukrainian].
  17. 17. Kostyshyn NM, Gzhegotskyi MR, Servetnyk MI. Evaluation of structural and functional state of rat's bone tissue under whole body vibration. Ukrainian Journal of Occupational Health. 2017;(2):37-45.
  18. 18. Kostyshyn NM, Kostyshyn LP, Servetnyk MI, Gzhegotskyi MR. The Peculiarities of Remodelling Muscle Tissue of Rats Under the Vibration Influence. prilozi, 2019;40(1):59-65.
  19. 19. Clark SM, Iball J. The x-ray crystal analysis of bone. Progress in Biophysics and Bio- physical Chemistry: Progress Series. 2016;7:226.
  20. 20. Bunaciu AA, UdriŞTioiu EG, Aboul-Enein HY. X-ray diffraction: instrumentation and applications. Critical reviews in analytical chemistry. 2015;45(4):289-99.
  21. 21. Rogers KD, Daniels P. An X-ray diffraction study of the effects of heat treatment on bone mineral microstructure. Biomaterials. 2002;23(12):2577-85.
  22. 22. Piga G, Solinas G, Thompson TJU, Brunetti A, Malgosa A, Enzo S. Is X-ray diffrac- tion able to distinguish between animal and human bones? Journal of archaeological science. 2013;40(1):778-85.
  23. 23. Tadano S, Giri B. X-ray diffraction as a promising tool to characterize bone nanocom- posites. Science and technology of advanced materials. 2012;12(6):064708.

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