online ISSN 2415-3176
print ISSN 1609-6371
logoExperimental and Clinical Physiology and Biochemistry
  • 4 of 6
Up
ECPB 2020, 91(3): 26–29
https://doi.org/10.25040/ecpb2020.03-04.026
Research articles

Characteristic of changes in the blood transaminases activity in the dynamic of the combined pathology – immobilization stress and adrenaline-induced myocardial injury and their correction of L-arginine

O.B. LYS, O.I. HRUSHKA
Abstract

Alanine aminotransferase and aspartate aminotransferase – are the most authentic signs of cells damage and liver necrosis. Elevated levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) are found not only due to the hepatopathy, but also by cardiovascular and endocrine systems. The primary task of arginine in body is to be the substrate for nitric oxide synthesis that plays a crucial role in body under pathological and physiological conditions. Clinical effectiveness of L-arginine in clinical hepatology is beyond any doubt. Nowadays, there are unresolved questions that have to do with changes in the blood transaminase activity under the conditions of immobilization stress and adrenaline-induced myocardial injury as well as the effect of the drug L-arginine on them.

The aim of the study was to investigate the characteristics of changes in the blood transaminase activity with combined pathology of immobilization stress and adrenaline-induced myocardial injury. Furthermore, to establish corrective actions of L-arginine on them.

Experiments were carried out on white male rats of the Wistar line weighing (180-200 g), which were allocated into 4 groups of 10 animals (one control and three normal).

This study showed that on the first and third days under the conditions of immobilization stress and adrenaline-induced myocardial injury occurred activity elevation of aspartate aminotransferase in blood accordingly by 118.7% and 77.1% along with alanine aminotransferase by 290.0% and 255.0% respectively as compared to control rats.

At a subsequent time, on the fifth day under the conditions of immobilization stress and adrenaline-induced myocardial injury was concluded further activity elevation of aspartate aminotransferase in blood accordingly by 227.5% respectively as compared to intact group of animals.

Conducted biochemical research of alanine aminotransferase and aspartate aminotransferase demonstrated their increased activity in blood, especially on the first day of experiment under the conditions of combined impact of adrenaline and stress in the course of the full experimental period. It proved the cells damage and escape from them into the blood of these enzymes.

Comparing result analysis of the research before and after L-arginine treatment for animals under the conditions of immobilization stress and adrenaline-induced myocardial injury showed that after using L-arginine on the fifth day of experiment we have got a decrease level index in alanine aminotransferase in blood by 175.0% and aspartate aminotransferase in blood by 52.0% (Fig. 2). It can be concluded that this amino acid as the hepatoprotector and anti-oxidant has a good effect on the blood transaminase activity index under the conditions of combined pathology of immobilization stress and adrenaline-induced myocardial injury.

From these facts, one may conclude that L-arginine would be reasonable to use as the hepatoprotector and anti-oxidant that has a good effect on the blood transaminase activity index under the conditions of combined pathology of immobilization stress and adrenaline-induced myocardial injury.

Recieved: 09.11.2020

Keywords: alanine aminotransferase, aspartate aminotransferase, immobilization stress, adrenaline-induced myocardial injury, L-arginine

Full text: PDF (Ukr) 493K

References
  1. 1. Babak OY Clinical significance and diagnostic tactics in the increase of serum transaminases in the absence of clinical manifestations. Medical Journal; The Art of Healing. 2006; 8 (34): 1
  2. 2.Shugalei VS, Ananyan AA. Arginine regulation of cytochrome P450 activity and lipid peroxidation in the liver and testes of rats during hypoxia. Medical chemistry issues. 1991; 4: 51-54
  3. 3. Stepanov YM, Kononov IN, Zhurbina AI, Filippova AY. Arginine in medical practice (Literature review). Suchas. gastroenterology. 2005; 4: 121-127
  4. 4. Wu G, Meininger CJ. Arginine nutrition and cardiovascular function J. Nutr. 2000. 130: 2626-2629.
  5. 5. Rector TS, Bank AJ, Mullen KA, Tschumperlin LK. Randomized, double-blind, placebo-controlled study of supplemental oral L-arginine in patients with heart failure. Circulation. 1997. 18: 1674-1679.
  6. 6. Almakaeva LG, Litvinova EV Arginine and its application in information medicine. Medicines of Ukraine. 2011; 1 (5): 23-26
  7. 7. Reitman S, Frankel S. Colorimetric method for the determination of glutamicoxaloaceticand glutamic pyruvic transaminases. Am. J. Clin. Pathol. 1957; 28: 56.
  8. 8. Gorizontov PD, Belousova OI, Fetodov MI Stress and the blood system. M .: Medicine.1983; 338.
  9. 9. Markova OO Myocardial infarction and reactivity of the organism. Ternopil Ukrmedknyha. 1998; 152.
  10. 10. Tkach EI Storozhuk VP General theory of statistics. Kiev; Center for Educational Literature ;. 2009; 440


Програмування - Roman.im