We have previously shown that the immune responses of female rats to chronic immobilization-aversion stress are ambiguous and can be grouped into at least three clusters. The aim of this study is to identify the peculiarities of metabolism in different variants of the immune response to chronic stress in the same animals. Material and methods. The experiment was performed on 60 healthy Wistar female rats weighing 220-300 g. 10 animals remained intact, and the other rats 6 days subjected to moderate stress by daily 30-minute immobilization. The day after the completion of the stressing course we determined the plasma levels of the electrolytes (calcium, magnesium, phosphates, potassium); nitrogenous metabolites (creatinine, urea, uric acid, bilirubin); middle mass molecules, amylase, glucose, cholesterol as well as lipids peroxidation parameters (diene conjugates, malonic dialdehyde, catalase plasma and superoxide dismutase erythrocytes).The level of sodium determined in erythrocytes. Most of the listed parameters of metabolism were also determined in daily urine. Glomerular filtration, canalicular reabsorption and lithogenicity of urine were calculated. Results. Based on the discriminant analysis by the forward stepwise method, 22 parameters are included in the model. Members of first cluster with minimal stress induced immune dysfunction are characterized by higher than normal levels of glomerulary filtration, diurese, phosphates excretion, calcium urine and malonic dialdehyde plasma, and normal levels of body mass, potassium urine and magnesium plasma but maximum among stressed animals, as well as lower than normal activity of superoxide dismutase and normal but minimal for sampling levels of canalicular reabsorbtion, uric acid plasma and sodium erythrocytes. The members of the second cluster with maximal immune dysfunction are characterized by decreased/increased or minimum/maximum levels of the listed parameters of metabolism. The members of the third cluster are characterized by higher than normal plasma levels of phosphate, creatinine, urea and amylase, as well as lower than normal urine levels of uric acid, middle mass molecules, diene conjugates and lythogenicity urine as well as normal but maximal for sampling levels of katalase urine and diene conjugates plasma. Overall classification accuracy is 92%. Conclusion. The previously identified three variants of immune responses to chronic stress are accompanied by characteristic constellations of metabolic parameters.
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