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ECPB 2018, 84(4): 5–10
https://doi.org/10.25040/ecpb2018.04.005
Research articles

Serum Amino Acid Levels in Rats under Long-term Administration of Progesterone and Melanin Treatment

A.V. ALEKSANDROV, V.V. KONOPELNIUK, O.V. SKOPENKO, I.V. KOMPANETS, L.I. OSTAPCHENKO
Abstract

Background. Obesity is one of the most common complex health problems in the world. Main reasons of the development of obesity are marketing the unhealthy food, passive lifestyle, hormonal disorders. Obesity can lead to the development of diabetes mellitus, ischemic heart disease and certain cancers. Numerous neuropeptides and transmitters as well as multiple peripheral hormones take part not only in the regulation of feeding but also in the physical activity. Peptides - serotonin, insulin, glucagon, hormones of hypothalamus and hypophysis, thyroid hormones are the examples of weight regulated hormones. The use of drugs based on steroid hormones, particularly progesterone, can also be one of many factors contributing to obesity. It is known that progesterone which enters the female body artificially leads to fat accumulation. Phenolic biopolymers are the most thoroughly studied natural genesis of macromolecular structures. Melanin occupies a special place among natural polymers. Melanin is chemically relatively inert, but at the same time it is the only polymer with biological properties of a stable free radical. These characteristics determine the structure of their photo- and radioprotective, antioxidant and other properties that is why melanin is widely used in medicine, pharmacology, cosmetology, agriculture and food industry. This article is focused on the molecular and clinical associations between melanin and serum amino acids under progesterone-induced obesity. Serum amino acids are a source for synthesis of hormones that have peptide and protein nature. Revealing these interactions and mechanisms of progesterone-induced obesity may improve our understanding of the complex treatment of obesity. Materials and methods. In the current study we used white nonlinear female rats that were divided into three groups. Rats of group 1 – control; group 2 – progesterone-induced obese; group 3 – progesterone-induced obese group under melanin treatment (10 mg/kg body weight, treated daily during 28 days). Amino acid blood serum levels were determined by ion exchange chromatography analyzer (Spekman, Stein, Moore). The statistical analysis of data was carried out by the software package "Statistica 7.0". Values are statistically significant at p < 0.05. Results. The recent study has shown changes in amino acid levels in blood serum of rats under progesterone long-term administration and melanin treatment. Levels of the majority of amino acid content have decreased in rats under progesterone long-term administration and melanin treatment as compared with the control group. The obtained data have given some evidence that progesterone long-term administration has a significant influence on amino acids metabolism. Conclusion. Aromatic amino acids are the substrates for the synthesis of neurotransmitters. Changes of content of aromatic amino acids can be a reason of disfunction of the synthesis of these neurotransmitters. The significant decrease of some amino acids, particularly BCAAs and aromatic amino acids, can show disorders of transport processes and metabolism of amino acids. This, directly or indirectFly, leads to weight disorders. Article recieved: 31.10.2018

Keywords: amino acids, melanin, progesterone, obesity

Full text: PDF (Eng) 323K

References
  1. 1. Chan M. Obesity and diabetes: the slow-motion disaster Keynote address at the 47th meet- ing of the National Academy of Medicine. The Milbank quarterly. A multidisciplinary journal of population health and health policy. Washington 2017;95(1):11-4.
  2. 3. Gautron L, Elmquist JK, Williams KW. Neural Control of Energy Balance: Translating Circuits to Therapies. Cell. 2015;161:133-45. doi.org/10.1016/j.cell.2015.02.023
  3. 4. Practice Committee of the American Society for Reproductive Medicine. Obesity and reproduction: a committee opinion. Fertil Steril. 2015;104:1116-26. doi.org/10.1016/j.fertnstert.2015.08.018
  4.  5. Scott M, Xu Y, Elias C, Kevin W. Williams. Central regulation of food intake, body weight, energy expenditure, and glucose homeostasis. Front Neuroscience. 2014;8:384. doi.org/10.3389/fnins.2014.00384
  5. 6. Loh K, Herzog H, Shi Y. Regulation of energy homeostasis by the NPY system. Trends in Endocrinology and Metabolism. 2015; 26: 125-35. doi.org/10.1016/j.tem.2015.01.003
  6.  7. Jain A, Polotsky A, Rochester D, Berga S, Loucks T, Zeitlian G et al. Pulsatile luteinizing hormone amplitude and progesterone metabolite excretion are reduced in obese women. The Journal of Clinical Endocrinology & Metabolism. 2007;92:2468-73. doi.org/10.1210/jc.2006-2274
  7. 8. Santoro N, Lasley B, McConnell D, Allsworth J, Crawford S, Gold E et al. Body size and ethnicity are associated with menstrual cycle alterations in women in the early menopausal transition: The Study of Women's Health across the Nation (SWAN) Daily Hormone Study. The Journal of Clinical Endocrinology and Metabolism. 2004;89:2622-31. doi.org/10.1210/jc.2003-031578  
  8. 9. Avetisyan S, Hovsepyan A, Chakhalyan A, Keleshyan S, Aghajanyan A, Saghiyan A. Study of the influence of the composition of fermentation medium and fermentation conditions on melanin biosynthesis by Bacillus thuringiensis K1 strain. Electronic Journal of Natural Sciences. 2009;2(13):24-7.
  9. 10. Zheng Y, Ceglarek U, Huang T, Lerong Li, Rood J, Donna H. Ryan et al. Weight-loss diets and 2-y changes in circulating amino acids in 2 randomized intervention trials. The American Journal of Clinical Nutrition. 2016;103(2):505-11. doi.org/10.3945/ajcn.115.117689
  10. 11. Cota D, Proulx K, Smith K, Kozma S, Thomas G, Woods S et al. Hypothalamic mTOR signaling regulates food intake. Science. 2006;312:927-30. doi.org/10.1126/science.1124147
  11. 12. Torres-Leal F, Fonseca-Alaniz M, Teodoro G, de Capitani M, Vianna D, Pantaleão L et.al. Leucine supplementation improves adiponectin and total cholesterol concentrations despite the lack of changes in adiposity or glucose homeostasis in rats previously exposed to a high-fat diet. Nutrition Metabolism. 2011;8:62. doi.org/10.1186/1743-7075-8-62
  12. 13. Wang T, Larson M, Vasan R, Cheng S, Phee E, McCabe E et al. Metabolite profiles and the risk of developing diabetes. Nature Medicine 2011;17:448-54. doi.org/10.1038/nm.2307
  13. 14. Fei Xiao, Ying Du, Ziquan Lv, Shanghai Chen, Jianmin Zhu, Hongguang Sheng et al. Essential amino acids and lipid metabolism. Society for Endocrinology 2016;57(4):223-31.
  14. 15. Tsikas D, Guoyao Wu. Homoarginine, arginine and relatives: analysis, metabolism, transport, physiology and pathology. Amino Acids. 2015;47:1697-702. doi.org/10.1007/s00726-015-2055-5
  15. 16. Bi X, Henry C. Plasma-free amino acid profiles are predictors of cancer and diabetes development. Nutrition and Diabetes. 2017;7(249):1-9. doi.org/10.1038/nutd.2016.55
  16. 17. Belemets N, Kobyliak N, Virchenko O, Falalyeyeva T, Olena T, Bodnar P. et al. Effects of polyphenol compounds melanin on NAFLD/NASH prevention. Biomedicine and Pharmaco- therapy. 2017;88:267-76. doi.org/10.1016/j.biopha.2017.01.028


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