DPhysical methods of influencing a wound defect are widely used in the process of treating injuries. The aim of our work was to study the effect of photobiomodulation (PBM) therapy on the regulation of disorders of chronic wound healing by cytokines (for example, interleukin-1β (IL-1β) and interleukin-4 (IL-4)). The study involved 54 Wistar rats: intact animals (6 rats), animals of the control and experimental groups (24 rats in each group), which were simulated chronic wounds. The rats of the experimental group received PBM therapy once a day for 5 days. The laser apparatus Lika-therapist M (Cherkassy) was used in a continuous mode at a wavelength of 660 nm, an output power of 10 mW, an energy density of 1 J/cm 2 , and an exposure time of 314 s. The wounds of the animals in the control group treated with sham. The rats were taken out of the experiment on days 3, 7, 14, and 28 after surgery. The study of the levels of IL-1β and IL-4 in the blood serum of animals was carried out by the method of enzyme-linked immunosorbent assay. The use of PBM therapy led to an increase in IL-1β levels 3 days after wound induction and to a decrease after 28 days. IL-4 concentrations decreased after 3 days and increased after 14 and 28 days in the group of animals whose wound defects were exposed to PBM therapy. The use of PBM therapy contributes to the regulation of chronic wound healing processes by modulating the levels of IL-1β and IL-4 at different stages of healing. A better understanding of the cellular and molecular mechanisms underlying wound healing with PBM therapy will allow influencing the repair processes, which will lead to new and effective therapeutic strategies for many pathological conditions.

Keywords: chronic wound, photobiomodulation therapy, cytokines, experimental model

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